Nup133 Antibody (E-4): sc-376699
- Nup133 Antibody (E-4) is a mouse monoclonal IgG1 κ, cited in 4 publications, provided at 200 µg/ml
- raised against amino acids 813-1156 mapping at the C-terminus of Nup133 of human origin
- Anti-Nup133 Antibody (E-4) is recommended for detection of Nup133 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-Nup133 Antibody (E-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgGκ BP-HRP is the preferred secondary detection reagent for Nup133 Antibody (E-4) for WB applications. This reagent is now offered in a bundle with Nup133 Antibody (E-4) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
Nup133 Antibody (E-4) is a mouse monoclonal IgG1 kappa light chain antibody that detects Nup133 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-Nup133 antibody (E-4) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Nup133, a crucial component of nuclear pore complexes (NPCs), plays a significant role in the transport of macromolecules between the nucleus and cytoplasm, which is vital for maintaining cellular function and gene expression regulation. Nup133 is strategically located on both the cytoplasmic and nuclear sides of the nuclear pore, and during mitosis, localizes to kinetochores, highlighting its importance in cell division. Nup133 is part of the Nup160 nuclear pore subcomplex, which includes Nup160, Nup96, and Nup107, and is essential for RNA export, ensuring that mRNA and other RNA species are efficiently transported out of the nucleus to the cytoplasm for translation. The presence of O-linked N-acetylglucosamine moieties and a distinctive pentapeptide repeat (XFXFG) in nucleoporins, including Nup133, underscores the complexity and regulatory mechanisms involved in nucleocytoplasmic transport, making Nup133 (E-4) antibody a valuable tool for researchers studying cellular transport processes and their implications in health and disease.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Nup133 Antibody (E-4) References:
- Sequential recruitment of NPC proteins to the nuclear periphery at the end of mitosis. | Bodoor, K., et al. 1999. J Cell Sci. 112 (Pt 13): 2253-64. PMID: 10362555
- An evolutionarily conserved NPC subcomplex, which redistributes in part to kinetochores in mammalian cells. | Belgareh, N., et al. 2001. J Cell Biol. 154: 1147-60. PMID: 11564755
- Novel vertebrate nucleoporins Nup133 and Nup160 play a role in mRNA export. | Vasu, S., et al. 2001. J Cell Biol. 155: 339-54. PMID: 11684705
- The conserved Nup107-160 complex is critical for nuclear pore complex assembly. | Walther, TC., et al. 2003. Cell. 113: 195-206. PMID: 12705868
- Structural and functional analysis of Nup133 domains reveals modular building blocks of the nuclear pore complex. | Berke, IC., et al. 2004. J Cell Biol. 167: 591-7. PMID: 15557116
- Architectural nucleoporins Nup157/170 and Nup133 are structurally related and descend from a second ancestral element. | Whittle, JRR. and Schwartz, TU. 2009. J Biol Chem. 284: 28442-28452. PMID: 19674973
- Cell cycle-dependent differences in nuclear pore complex assembly in metazoa. | Doucet, CM., et al. 2010. Cell. 141: 1030-41. PMID: 20550937
- Analysis of the initiation of nuclear pore assembly by ectopically targeting nucleoporins to chromatin. | Schwartz, M., et al. 2015. Nucleus. 6: 40-54. PMID: 25602437
- Crystal structure of human nuclear pore complex component NUP43. | Xu, C., et al. 2015. FEBS Lett. 589: 3247-53. PMID: 26391640
- Biallelic Mutations in Nuclear Pore Complex Subunit NUP107 Cause Early-Childhood-Onset Steroid-Resistant Nephrotic Syndrome. | Miyake, N., et al. 2015. Am J Hum Genet. 97: 555-66. PMID: 26411495
- The role of the FSGS disease gene product and nuclear pore protein NUP205 in regulating nuclear localization and activity of transcriptional regulators YAP and TAZ. | Ester, L., et al. 2023. Hum Mol Genet. 32: 3153-3165. PMID: 37565816
- Sequence analysis of a cDNA encoding a human nuclear pore complex protein, hnup153. | McMorrow, I., et al. 1994. Biochim Biophys Acta. 1217: 219-23. PMID: 8110839
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Nup133 Antibody (E-4) | sc-376699 | 200 µg/ml | $316.00 | |||
Nup133 Antibody (E-4): m-IgGκ BP-HRP Bundle | sc-523051 | 200 µg Ab, 40 µg BP | $354.00 | |||
Nup133 Antibody (E-4) AC | sc-376699 AC | 500 µg/ml, 25% agarose | $416.00 | |||
Nup133 Antibody (E-4) HRP | sc-376699 HRP | 200 µg/ml | $316.00 | |||
Nup133 Antibody (E-4) FITC | sc-376699 FITC | 200 µg/ml | $330.00 | |||
Nup133 Antibody (E-4) PE | sc-376699 PE | 200 µg/ml | $343.00 | |||
Nup133 Antibody (E-4) Alexa Fluor® 488 | sc-376699 AF488 | 200 µg/ml | $357.00 | |||
Nup133 Antibody (E-4) Alexa Fluor® 546 | sc-376699 AF546 | 200 µg/ml | $357.00 | |||
Nup133 Antibody (E-4) Alexa Fluor® 594 | sc-376699 AF594 | 200 µg/ml | $357.00 | |||
Nup133 Antibody (E-4) Alexa Fluor® 647 | sc-376699 AF647 | 200 µg/ml | $357.00 | |||
Nup133 Antibody (E-4) Alexa Fluor® 680 | sc-376699 AF680 | 200 µg/ml | $357.00 | |||
Nup133 Antibody (E-4) Alexa Fluor® 790 | sc-376699 AF790 | 200 µg/ml | $357.00 |