NUBP1 Antibody (H-4): sc-398991

NUBP1 Antibody (H-4) is a mouse monoclonal IgG1 antibody that detects NUBP1 in human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). NUBP1, also known as cytosolic Fe-S cluster assembly factor NUBP1 or Nbp35, is a crucial 320 amino acid protein that plays a significant role in the regulation of centrosome duplication, which is vital for proper cell division and maintaining genomic stability. Anti-NUBP1 antibody (H-4) recognizes a member of the Mrp/NBP35 ATP-binding protein family that exists in two alternatively spliced isoforms, allowing for functional diversity. NUBP1 is integral to the cytosolic iron-sulfur (Fe/S) protein assembly machinery, facilitating the transfer of iron-sulfur clusters to specific apoproteins, which is essential for the proper functioning of various enzymes and proteins that rely on these clusters for their activity. The gene encoding NUBP1 is located on human chromosome 16, a region associated with several genetic disorders, including giant axonal neuropathy and Rubinstein-Taybi syndrome, highlighting the importance of NUBP1 in cellular health and disease. NUBP1 (H-4) antibody is an invaluable tool for researchers investigating the intricate roles of this protein in cellular processes and its implications in human health.

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NUBP1 Antibody (H-4) References:

1. Two novel mouse genes--Nubp2, mapped to the t-complex on chromosome 17, and Nubp1, mapped to chromosome 16--establish a new gene family of nucleotide-binding proteins in eukaryotes.  |  Nakashima, H., et al. 1999. Genomics. 60: 152-60. PMID: 10486206
2. Determining the structure and mechanism of the human multidrug resistance P-glycoprotein using cysteine-scanning mutagenesis and thiol-modification techniques.  |  Loo, TW. and Clarke, DM. 1999. Biochim Biophys Acta. 1461: 315-25. PMID: 10581364
3. Insights into the structure and substrate interactions of the P-glycoprotein multidrug transporter from spectroscopic studies.  |  Sharom, FJ., et al. 1999. Biochim Biophys Acta. 1461: 327-45. PMID: 10581365
4. Structure and association of ATP-binding cassette transporter nucleotide-binding domains.  |  Kerr, ID. 2002. Biochim Biophys Acta. 1561: 47-64. PMID: 11988180
5. Human Nbp35 is essential for both cytosolic iron-sulfur protein assembly and iron homeostasis.  |  Stehling, O., et al. 2008. Mol Cell Biol. 28: 5517-28. PMID: 18573874
6. Comparison of intracellular localization of Nubp1 and Nubp2 using GFP fusion proteins.  |  Okuno, T., et al. 2010. Mol Biol Rep. 37: 1165-8. PMID: 19263241
7. Nubp1 is required for lung branching morphogenesis and distal progenitor cell survival in mice.  |  Schnatwinkel, C. and Niswander, L. 2012. PLoS One. 7: e44871. PMID: 23028652
8. Cystic fibrosis transmembrane conductance regulator (ABCC7) structure.  |  Hunt, JF., et al. 2013. Cold Spring Harb Perspect Med. 3: a009514. PMID: 23378596
9. GLRX3 Acts as a [2Fe-2S] Cluster Chaperone in the Cytosolic Iron-Sulfur Assembly Machinery Transferring [2Fe-2S] Clusters to NUBP1.  |  Camponeschi, F., et al. 2020. J Am Chem Soc. 142: 10794-10805. PMID: 32429669
10. Unraveling the mechanism of [4Fe-4S] cluster assembly on the N-terminal cluster binding site of NUBP1.  |  Bargagna, B., et al. 2023. Protein Sci. 32: e4625. PMID: 36916754

The preferred NUBP1 antibody is NUBP1 Antibody (C-3): sc-398368.