Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
NTR1 Double Nickase Plasmid (h): sc-402552-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- NTR1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- NTR1 Double Nickase Plasmid (h) and NTR1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting NTSR1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: NTR1 Antibody (B-12): sc-376958
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
NTR1 Double Nickase Plasmid (h) | sc-402552-NIC | 20 µg | $410.00 | |||
NTR1 Double Nickase Plasmid (h2) | sc-402552-NIC-2 | 20 µg | $410.00 |
NTSR1 encodes neurotensin receptor 1 (NTR1), a class A GPCR that binds the neuropeptide neurotensin to regulate intracellular signaling through Gαq/PLCβ-driven Ca²⁺ mobilization and PKC activation, with additional coupling to MAPK/ERK and β-arrestin–mediated trafficking. NTR1 influences neurotransmission, smooth muscle contraction, and secretory processes via receptor desensitization, internalization, and cross-talk with other GPCR pathways. Altered NTSR1 expression and signaling have been associated with dysregulated proliferative and inflammatory programs in multiple disease-relevant contexts, making it a useful node for mechanistic studies of GPCR signaling dynamics. As a membrane receptor with rapid ligand-induced endocytosis, NTR1 is also widely used to interrogate receptor pharmacology, compartmentalized signaling, and downstream transcriptional responses.
NTR1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the NTSR1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within NTSR1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt NTSR1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of NTSR1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.