Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
NTCP Double Nickase Plasmid (h): sc-400840-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- NTCP Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- NTCP Double Nickase Plasmid (h) and NTCP Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting SLC10A1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: NTCP Antibody (D-5): sc-518115
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
NTCP Double Nickase Plasmid (h) | sc-400840-NIC | 20 µg | $410.00 | |||
NTCP Double Nickase Plasmid (h2) | sc-400840-NIC-2 | 20 µg | $410.00 |
SLC10A1 encodes the sodium/taurocholate cotransporting polypeptide (NTCP), a hepatocyte basolateral membrane transporter that mediates sodium-dependent uptake of conjugated bile acids and other organic anions from portal blood. NTCP activity is integral to enterohepatic bile acid circulation and influences intracellular bile acid–activated signaling networks, including FXR-regulated transcriptional programs that couple transport to metabolic homeostasis. Altered SLC10A1/NTCP function can perturb bile acid handling and hepatic physiology, providing a mechanistic link to cholestatic phenotypes and broader dysregulation of lipid and glucose metabolism. NTCP also serves as a host factor for pathogen entry into hepatocytes, making it relevant for studies of liver-tropic infection biology and receptor-mediated uptake processes.
NTCP Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the SLC10A1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within SLC10A1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt SLC10A1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of SLC10A1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.