NTAL Antibody (D-10): sc-271000
- NTAL Antibody (D-10) is a mouse monoclonal IgG1 κ NTAL antibody provided at 200 µg/ml
- raised against a peptide mapping within a cytoplasmic domain of NTAL of rat origin
- NTAL Antibody (D-10) is recommended for detection of NTAL of mouse and rat origin by WB, IP, IF and ELISA
- Anti-NTAL Antibody (D-10) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP is the preferred secondary detection reagent for NTAL Antibody (D-10) for WB applications. This reagent is now offered in a bundle with NTAL Antibody (D-10) (see ordering information below).
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SEE ALSO...
NTAL Antibody (D-10) is a mouse monoclonal IgG1 kappa light chain antibody that detects NTAL protein of mouse and rat origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). NTAL Antibody (D-10) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The non-T cell activation linker (NTAL), also known as the membrane-associated adapter molecule, Williams-Beuren syndrome chromosome region 15 protein, or linker of activated B cells, plays a crucial role in immune signaling. NTAL is primarily located in membrane microdomains, or lipid rafts, of B cells, natural killer cells, and myeloid cells, as well as in monocytes and mast cells, but is absent in resting T lymphocytes. This strategic localization is vital for NTAL function, as NTAL becomes rapidly tyrosine-phosphorylated upon cross-linking of the B cell receptor or high-affinity Fc γ and Fc epsilon receptors, facilitating association with cytoplasmic signaling molecules that propagate immune responses. NTAL is highly expressed in the spleen, lymph node germinal centers, and peripheral blood lymphocytes, underscoring NTAL importance in the immune system. Notably, defects in the gene encoding NTAL can lead to the musculoskeletal and cardiovascular abnormalities associated with the rare developmental disorder Williams-Beuren syndrome.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
NTAL Antibody (D-10) References:
- Comparative genomic sequence analysis of the Williams syndrome region (LIMK1-RFC2) of human chromosome 7q11.23. | Martindale, DW., et al. 2000. Mamm Genome. 11: 890-8. PMID: 11003705
- LAB: a new membrane-associated adaptor molecule in B cell activation. | Janssen, E., et al. 2003. Nat Immunol. 4: 117-23. PMID: 12514734
- The importance of three membrane-distal tyrosines in the adaptor protein NTAL/LAB. | Koonpaew, S., et al. 2004. J Biol Chem. 279: 11229-35. PMID: 14722116
- NTAL phosphorylation is a pivotal link between the signaling cascades leading to human mast cell degranulation following Kit activation and Fc epsilon RI aggregation. | Tkaczyk, C., et al. 2004. Blood. 104: 207-14. PMID: 15010370
- Transmembrane adaptor molecules: a new category of lymphoid-cell markers. | Tedoldi, S., et al. 2006. Blood. 107: 213-21. PMID: 16160011
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
NTAL Antibody (D-10) | sc-271000 | 200 µg/ml | $316.00 | |||
NTAL Antibody (D-10): m-IgG Fc BP-HRP Bundle | sc-525777 | 200 µg Ab; 10 µg BP | $354.00 | |||
NTAL Antibody (D-10) AC | sc-271000 AC | 500 µg/ml, 25% agarose | $416.00 | |||
NTAL Antibody (D-10) HRP | sc-271000 HRP | 200 µg/ml | $316.00 | |||
NTAL Antibody (D-10) FITC | sc-271000 FITC | 200 µg/ml | $330.00 | |||
NTAL Antibody (D-10) PE | sc-271000 PE | 200 µg/ml | $343.00 | |||
NTAL Antibody (D-10) Alexa Fluor® 488 | sc-271000 AF488 | 200 µg/ml | $357.00 | |||
NTAL Antibody (D-10) Alexa Fluor® 546 | sc-271000 AF546 | 200 µg/ml | $357.00 | |||
NTAL Antibody (D-10) Alexa Fluor® 594 | sc-271000 AF594 | 200 µg/ml | $357.00 | |||
NTAL Antibody (D-10) Alexa Fluor® 647 | sc-271000 AF647 | 200 µg/ml | $357.00 | |||
NTAL Antibody (D-10) Alexa Fluor® 680 | sc-271000 AF680 | 200 µg/ml | $357.00 | |||
NTAL Antibody (D-10) Alexa Fluor® 790 | sc-271000 AF790 | 200 µg/ml | $357.00 | |||
NTAL (D-10) Neutralizing Peptide | sc-271000 P | 100 µg/0.5 ml | $68.00 |