NSUN5 Antibody (H-10): sc-376147
- NSUN5 Antibody (H-10) is a mouse monoclonal IgG1 κ NSUN5 antibody, cited in 7 publications, provided at 200 µg/ml
- raised against amino acids 227-365 mapping near the C-terminal of NSUN5 of human origin
- NSUN5 Antibody (H-10) is recommended for detection of NSUN5 of human origin by WB, IP, IF and ELISA
- Anti-NSUN5 Antibody (H-10) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP is the preferred secondary detection reagent for NSUN5 Antibody (H-10) for WB applications. This reagent is now offered in a bundle with NSUN5 Antibody (H-10) (see ordering information below).
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NSUN5 Antibody (H-10) is a mouse monoclonal IgG1 kappa light chain antibody that detects NSUN5 of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). NSUN5 (H-10) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. NSUN5, also known as NOL1, p120, or NOL1R, is a 429 amino acid protein that belongs to the methyltransferase superfamily and is expressed ubiquitously, with particularly high levels in the heart, placenta, and skeletal muscle. NSUN5 functions as an S-adenosyl-L-methionine-dependent methyltransferase, essential for various cellular processes, including RNA modification and gene regulation. Absence of NSUN5 has been implicated in the pathogenesis of Williams syndrome, highlighting its importance in normal development and function. NSUN5 undergoes post-translational modifications, including phosphorylation, likely mediated by ATM or ATR, which can influence activity and interactions within the cell. The gene encoding NSUN5 is located on human chromosome 7, a region associated with several genetic disorders, including Osteogenesis imperfecta and Shwachman-Diamond syndrome, underscoring the significance of NSUN5 in human health and disease.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
NSUN5 Antibody (H-10) References:
- Williams-Beuren syndrome: genes and mechanisms. | Francke, U. 1999. Hum Mol Genet. 8: 1947-54. PMID: 10469848
- Characterization of two novel genes, WBSCR20 and WBSCR22, deleted in Williams-Beuren syndrome. | Doll, A. and Grzeschik, KH. 2001. Cytogenet Cell Genet. 95: 20-7. PMID: 11978965
- Identification of additional transcripts in the Williams-Beuren syndrome critical region. | Merla, G., et al. 2002. Hum Genet. 110: 429-38. PMID: 12073013
- Mutational mechanisms of Williams-Beuren syndrome deletions. | Bayés, M., et al. 2003. Am J Hum Genet. 73: 131-51. PMID: 12796854
- The neurobiology of Williams syndrome: cascading influences of visual system impairment? | Eckert, MA., et al. 2006. Cell Mol Life Sci. 63: 1867-75. PMID: 16810457
- Williams-Beuren syndrome diagnosis using fluorescence in situ hybridization. | Osborne, LR., et al. 2006. Methods Mol Med. 126: 113-28. PMID: 16930009
- The genomic basis of the Williams-Beuren syndrome. | Schubert, C. 2009. Cell Mol Life Sci. 66: 1178-97. PMID: 19039520
- Molecular anatomy of chromosome 7q deletions in myeloid neoplasms: evidence for multiple critical loci. | Liang, H., et al. 1998. Proc Natl Acad Sci U S A. 95: 3781-5. PMID: 9520444
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
NSUN5 Antibody (H-10) | sc-376147 | 200 µg/ml | $316.00 | |||
NSUN5 Antibody (H-10): m-IgG Fc BP-HRP Bundle | sc-526004 | 200 µg Ab; 10 µg BP | $354.00 | |||
NSUN5 Antibody (H-10) AC | sc-376147 AC | 500 µg/ml, 25% agarose | $416.00 | |||
NSUN5 Antibody (H-10) HRP | sc-376147 HRP | 200 µg/ml | $316.00 | |||
NSUN5 Antibody (H-10) FITC | sc-376147 FITC | 200 µg/ml | $330.00 | |||
NSUN5 Antibody (H-10) PE | sc-376147 PE | 200 µg/ml | $343.00 | |||
NSUN5 Antibody (H-10) Alexa Fluor® 488 | sc-376147 AF488 | 200 µg/ml | $357.00 | |||
NSUN5 Antibody (H-10) Alexa Fluor® 546 | sc-376147 AF546 | 200 µg/ml | $357.00 | |||
NSUN5 Antibody (H-10) Alexa Fluor® 594 | sc-376147 AF594 | 200 µg/ml | $357.00 | |||
NSUN5 Antibody (H-10) Alexa Fluor® 647 | sc-376147 AF647 | 200 µg/ml | $357.00 | |||
NSUN5 Antibody (H-10) Alexa Fluor® 680 | sc-376147 AF680 | 200 µg/ml | $357.00 | |||
NSUN5 Antibody (H-10) Alexa Fluor® 790 | sc-376147 AF790 | 200 µg/ml | $357.00 |