NSP3 Antibody (J-16.1): sc-100792

NSP3 Antibody (J-16.1) is a mouse monoclonal IgG1 kappa light chain antibody that detects NSP3 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). NSP3 Antibody (J-16.1) is available as non-conjugated anti-NSP3 antibody (J-16.1). NSP3, also known as novel SH2-containing protein 3, plays a crucial role in cellular signaling pathways, particularly those involving the Eph family of receptor tyrosine kinases, which are essential for various developmental processes such as cell migration and axon guidance. NSP3 is primarily located in the cytoplasm and is expressed in both embryonic and adult brain tissues, highlighting NSP3′s importance in neural development and function. NSP3 contains a Src homology 2 domain that specifically binds to a conserved tyrosine-phosphorylated motif in the juxtamembrane region of the EphB2 receptor, suggesting NSP3 may act as a critical mediator in the signaling cascade initiated by Eph receptors. Notably, NSP3 can be heavily tyrosine-phosphorylated in the presence of activated EphB2, indicating NSP3 may also serve as a substrate for EphB2 kinase activity. By linking activated Eph receptors to small Ras superfamily GTPases, NSP3 is integral to the regulation of cellular responses to Eph receptor signaling, making NSP3 a significant target for research in developmental biology and neurobiology.

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NSP3 Antibody (J-16.1) References:

1. A novel signaling intermediate, SHEP1, directly couples Eph receptors to R-Ras and Rap1A.  |  Dodelet, VC., et al. 1999. J Biol Chem. 274: 31941-6. PMID: 10542222
2. In vitro guidance of retinal ganglion cell axons by RAGS, a 25 kDa tectal protein related to ligands for Eph receptor tyrosine kinases.  |  Drescher, U., et al. 1995. Cell. 82: 359-70. PMID: 7634326
3. Eph family transmembrane ligands can mediate repulsive guidance of trunk neural crest migration and motor axon outgrowth.  |  Wang, HU. and Anderson, DJ. 1997. Neuron. 18: 383-96. PMID: 9115733
4. Lerk2 (ephrin-B1) is a collapsing factor for a subset of cortical growth cones and acts by a mechanism different from AL-1 (ephrin-A5).  |  Meima, L., et al. 1997. Mol Cell Neurosci. 9: 314-28. PMID: 9268508
5. The Eph family of receptors.  |  Pasquale, EB. 1997. Curr Opin Cell Biol. 9: 608-15. PMID: 9330863
6. The Eph family in the patterning of neural development.  |  Drescher, U. 1997. Curr Biol. 7: R799-807. PMID: 9382835
7. The ephrins and Eph receptors in neural development.  |  Flanagan, JG. and Vanderhaeghen, P. 1998. Annu Rev Neurosci. 21: 309-45. PMID: 9530499
8. Signaling by Eph receptors and their ephrin ligands.  |  Brückner, K. and Klein, R. 1998. Curr Opin Neurobiol. 8: 375-82. PMID: 9687349

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-NSP3 Antibody (J-16.1) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-NSP3 Antibody (J-16.1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

NSP3 Antibody (J-16.1) Specifications

Alternate Product Name: NSP3

Product Clone Name: J-16.1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-111

Epitope Target: NSP3

Epitope Species: human

Product Reactivity/Detection: SH2 domain of NSP3

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 100 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for NSP3 Antibody (J-16.1): N/A