NRAMP 1 CRISPR/Cas9 KO Plasmid (h): sc-402423

Overview

SLC11A1 encodes NRAMP1, a proton-coupled divalent metal transporter primarily associated with endosomal and phagosomal membranes in myeloid cells, where it regulates flux of Fe²⁺ and Mn²⁺ and shapes the metal availability within pathogen-containing compartments. By controlling intravesicular metal composition, NRAMP1 influences antimicrobial effector programs, redox balance, and innate immune signaling that intersect with inflammatory pathways and antigen handling. Genetic variation or altered expression of SLC11A1 has been linked to differences in susceptibility to intracellular infection and modulation of inflammatory phenotypes, making it a useful locus for host–pathogen and immune regulation studies. In human cell models, NRAMP1 function is often explored in the context of macrophage activation, phagosome maturation, and nutrient-limitation mechanisms that impact microbial persistence.

NRAMP 1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the SLC11A1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the SLC11A1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.

The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the SLC11A1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish NRAMP 1 protein expression.

This CRISPR knockout system enables efficient generation of SLC11A1-deficient cell models for investigation of NRAMP 1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.

Key Features

• sgRNAs targeting SLC11A1 exon(s) critical for NRAMP 1 function
• Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
• GFP reporter for identification of transfected cells
• Pool of plasmids targeting multiple SLC11A1 genomic sites to improve knockout efficiency
• Compatible with delivery by transfection

Design Variants

CRISPRs +/- HDRs

• gRNAs encoded by NRAMP 1 CRISPR/Cas9 KO Plasmid (h) and NRAMP 1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the SLC11A1 locus. One or both targeting designs may be available. See Related Products for availability.
• HDR donor constructs encoded by NRAMP 1 HDR Plasmid (h) and NRAMP 1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by SLC11A1 homology arms to support homology-directed repair at defined SLC11A1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.