Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
NR5A2 Double Nickase Plasmid (h): sc-402656-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- NR5A2 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- NR5A2 Double Nickase Plasmid (h) and NR5A2 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting NR5A2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: NR5A2 Antibody (B-8): sc-393369
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
NR5A2 Double Nickase Plasmid (h) | sc-402656-NIC | 20 µg | $410.00 | |||
NR5A2 Double Nickase Plasmid (h2) | sc-402656-NIC-2 | 20 µg | $410.00 |
NR5A2 (nuclear receptor subfamily 5 group A member 2; LRH-1) is an orphan nuclear receptor transcription factor that binds hormone response elements to regulate gene programs controlling cholesterol and bile acid homeostasis, steroidogenesis, and lipid metabolism. In epithelial contexts, NR5A2 also supports proliferative and differentiation states by integrating cues from nuclear receptor signaling with metabolic and developmental transcriptional networks. Its activity intersects with pathways governing pancreatic and hepatic function, and altered NR5A2 expression or regulatory variants have been linked to disease-relevant phenotypes including inflammation and cancer-associated transcriptional reprogramming. As a lineage and metabolism-associated regulator, NR5A2 is frequently studied in models of gastrointestinal, liver, and pancreatic biology, as well as in transcriptional circuitry analyses.
NR5A2 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the NR5A2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within NR5A2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt NR5A2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of NR5A2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.