NQO1 Antibody (A-5): sc-271116

NQO1 Antibody (A-5) is a mouse monoclonal IgG2a kappa light chain antibody that detects NQO1 of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). NQO1 Antibody (A-5) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. NAD(P)H:quinone oxidoreductase 1 (NQO1) plays a crucial role in cellular defense by catalyzing the reduction of quinones to hydroquinones, thereby detoxifying harmful compounds and protecting cells from oxidative stress, cytotoxicity, and mutagenicity. NQO1 is particularly important in cancer, as many tumors exhibit overexpression of NQO1, which functions as an obligate two-electron reductase that not only deactivates toxins but also activates bioreductive anticancer drugs, enhancing therapeutic efficacy. NQO1 is a 274 amino acid protein widely expressed across various tissues, although expression levels can vary significantly. NQO1 gene expression is tightly controlled and can be induced by factors such as xenobiotics, antioxidants, heavy metals, and radiation, with the antioxidant response element (ARE) in the NQO1 promoter playing a pivotal role in this process. Activation of the ARE by compounds like tert-butylhydroquinone is mediated through the PI3-kinase pathway, which is upstream of the transcription factor Nrf2. Various transcription factors, including Nrf2, c-Jun, Nrf1, Jun-B, and Jun-D, bind to the ARE to regulate NQO1 expression, while certain heterodimers, such as Maf-Nrf2, may negatively regulate this transcriptional activation.

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Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-NQO1 Antibody (A-5) has 34 citations in a variety of scientific publications.

Primary antibodies like Anti-NQO1 Antibody (A-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

NQO1 Antibody (A-5) Specifications

Alternate Product Name: NQO1

Product Clone Name: A-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 185-274

Epitope Target: NQO1

Epitope Species: human

Product Reactivity/Detection: NQO1

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 34 citations in various scientific publications

Available Conjugates/Forms for NQO1 Antibody (A-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred NQO1 antibody is NQO1 Antibody (A180): sc-32793.