NPY4-R CRISPR/Cas9 KO Plasmid (h): sc-406230
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- NPY4-R CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the NPY4-R genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
NPY4-R CRISPR/Cas9 KO Plasmid (h) | sc-406230 | 20 µg | $397.00 |
Overview
NPY4R encodes neuropeptide Y receptor Y4 (NPY4-R), a Gi/o-coupled GPCR preferentially activated by pancreatic polypeptide that modulates cAMP signaling, ion channel activity, and downstream MAPK/ERK pathways. NPY4-R is expressed in peripheral tissues and select CNS regions, linking neuroendocrine inputs to regulation of feeding behavior, energy balance, gastrointestinal motility, and autonomic control. Receptor activation influences cellular excitability and secretory programs through inhibition of adenylyl cyclase and βγ-mediated signaling. Altered NPY4R signaling has been studied in the context of metabolic physiology, obesity-related traits, and gastrointestinal functional regulation, providing a mechanistic entry point for interrogating neuropeptide-driven homeostatic circuits.
NPY4-R CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the NPY4R gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the NPY4R together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the NPY4R open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish NPY4-R protein expression.
This CRISPR knockout system enables efficient generation of NPY4R-deficient cell models for investigation of NPY4-R signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting NPY4R exon(s) critical for NPY4-R function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple NPY4R genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by NPY4-R CRISPR/Cas9 KO Plasmid (h) and NPY4-R CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the NPY4R locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by NPY4-R HDR Plasmid (h) and NPY4-R HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by NPY4R homology arms to support homology-directed repair at defined NPY4R target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.