NPY CRISPR/Cas9 KO Plasmid (h): sc-401098
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- NPY CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the NPY genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: NPY Antibody (F-6): sc-133080
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
NPY CRISPR/Cas9 KO Plasmid (h) | sc-401098 | 20 µg | $397.00 |
Overview
Neuropeptide Y (NPY) encodes a 36–amino acid secreted neuropeptide that signals through G protein–coupled Y receptors to regulate neurotransmission, neuroendocrine secretion, and autonomic control of energy balance. NPY activity couples to Gi/o-mediated signaling to modulate cAMP levels, calcium flux, and downstream kinase pathways that influence neuronal excitability, synaptic plasticity, and appetite-related circuitry. Beyond the central nervous system, NPY contributes to stress responses, vascular tone, and immune cell behavior, linking it to processes such as inflammation and metabolic homeostasis. Dysregulated NPY signaling has been studied in contexts including obesity and metabolic disease, anxiety- and stress-related phenotypes, and neurodegenerative or neuroinflammatory conditions.
NPY CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the NPY gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the NPY together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the NPY open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish NPY protein expression.
This CRISPR knockout system enables efficient generation of NPY-deficient cell models for investigation of NPY signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting NPY exon(s) critical for NPY function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple NPY genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by NPY CRISPR/Cas9 KO Plasmid (h) and NPY CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the NPY locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by NPY HDR Plasmid (h) and NPY HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by NPY homology arms to support homology-directed repair at defined NPY target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.