NPT2 CRISPR/Cas9 KO Plasmid (h): sc-402216
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- NPT2 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the NPT2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
NPT2 CRISPR/Cas9 KO Plasmid (h) | sc-402216 | 20 µg | $397.00 |
Overview
SLC34A1 encodes the human sodium-dependent phosphate transport protein 2A (NPT2), an apical membrane cotransporter that mediates electrogenic uptake of inorganic phosphate coupled to Na+ gradients in epithelial cells. NPT2 is a key determinant of phosphate homeostasis and participates in renal proximal tubule reabsorption pathways that intersect with vitamin D metabolism, parathyroid hormone signaling, and FGF23-regulated phosphate handling. Altered SLC34A1 function has been associated with disorders of phosphate balance, including phenotypes linked to nephrolithiasis, hypophosphatemia, and defects in mineralization, making it relevant for studying epithelial transport regulation and metabolic signaling. At the cellular level, SLC34A1 perturbation can be used to interrogate membrane trafficking, transporter kinetics, and phosphate-dependent control of bioenergetics and biomineral metabolism.
NPT2 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the SLC34A1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the SLC34A1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the SLC34A1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish NPT2 protein expression.
This CRISPR knockout system enables efficient generation of SLC34A1-deficient cell models for investigation of NPT2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting SLC34A1 exon(s) critical for NPT2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple SLC34A1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by NPT2 CRISPR/Cas9 KO Plasmid (h) and NPT2 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the SLC34A1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by NPT2 HDR Plasmid (h) and NPT2 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by SLC34A1 homology arms to support homology-directed repair at defined SLC34A1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.