NPRL2 Antibody (F-3): sc-376986

NPRL2 Antibody (F-3) is a mouse monoclonal IgG2a kappa light chain antibody that detects NPRL2 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-NPRL2 antibody (F-3) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. NPRL2, also known as tumor suppressor candidate 4 (TUSC4), is a 380 amino acid protein characterized by a bipartite nuclear localization signal and a granulin protein-binding domain, which are crucial for cellular processes. NPRL2 is predominantly expressed in skeletal muscle, with significant levels in brain, liver, and pancreas, while lower levels are found in lung, kidney, placenta, and heart. NPRL2 shows high expression in various lung cancer cell lines, suggesting a potential role in tumor suppression. NPRL2 participates in critical cellular mechanisms such as mismatch repair, cell cycle checkpoint signaling, and activation of apoptotic pathways, which are essential for maintaining genomic stability and preventing tumorigenesis. NPRL2 may enhance therapeutic efficacy of chemotherapy drugs like cisplatin by resensitizing resistant cancer cells, making NPRL2 a valuable target for cancer research and treatment strategies. NPRL2 gene is conserved across species and produces two isoforms due to alternative splicing, highlighting evolutionary significance and functional versatility.

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NPRL2 Antibody (F-3) References:

1. The 630-kb lung cancer homozygous deletion region on human chromosome 3p21.3: identification and evaluation of the resident candidate tumor suppressor genes. The International Lung Cancer Chromosome 3p21.3 Tumor Suppressor Gene Consortium.  |  Lerman, MI. and Minna, JD. 2000. Cancer Res. 60: 6116-33. PMID: 11085536
2. Expression of several genes in the human chromosome 3p21.3 homozygous deletion region by an adenovirus vector results in tumor suppressor activities in vitro and in vivo.  |  Ji, L., et al. 2002. Cancer Res. 62: 2715-20. PMID: 11980673
3. Functional characterization of the candidate tumor suppressor gene NPRL2/G21 located in 3p21.3C.  |  Li, J., et al. 2004. Cancer Res. 64: 6438-43. PMID: 15374952
4. Expression of candidate chromosome 3p21.3 tumor suppressor genes and down-regulation of BLU in some esophageal squamous cell carcinomas.  |  Yi Lo, PH., et al. 2006. Cancer Lett. 234: 184-92. PMID: 15885884
5. The 3p21.3 tumor suppressor NPRL2 plays an important role in cisplatin-induced resistance in human non-small-cell lung cancer cells.  |  Ueda, K., et al. 2006. Cancer Res. 66: 9682-90. PMID: 17018626
6. Simultaneous down-regulation of tumor suppressor genes RBSP3/CTDSPL, NPRL2/G21 and RASSF1A in primary non-small cell lung cancer.  |  Senchenko, VN., et al. 2010. BMC Cancer. 10: 75. PMID: 20193080
7. [Effect of recombinant plasmid pEGFP-N1-NPRL2 on the biological characteristics of human gastric cancer cells].  |  Hu, ZM., et al. 2012. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 28: 1020-4. PMID: 23046930
8. Nitrogen permease regulator-like 2 enhances sensitivity to oxaliplatin in colon cancer cells.  |  Liu, MN., et al. 2015. Mol Med Rep. 12: 1189-96. PMID: 25777765
9. Overexpression of Nitrogen Permease Regulator Like-2 (NPRL2) Enhances Sensitivity to Irinotecan (CPT-11) in Colon Cancer Cells by Activating the DNA Damage Checkpoint Pathway.  |  Liu, S. and Liu, B. 2018. Med Sci Monit. 24: 1424-1433. PMID: 29519997
10. PRMT1 orchestrates with SAMTOR to govern mTORC1 methionine sensing via Arg-methylation of NPRL2.  |  Jiang, C., et al. 2023. Cell Metab. 35: 2183-2199.e7. PMID: 38006878
11. NPRL2 is required for proliferation of oncogenic Ras-transformed bronchial epithelial cells.  |  Chuang, JY., et al. 2024. Cell Div. 19: 22. PMID: 38915098
12. NPRL2 promotes TRIM16-mediated ubiquitination degradation of Galectin-3 to prevent CD8+T lymphocyte cuproptosis in glioma.  |  Wang, F., et al. 2024. Cell Mol Life Sci. 81: 424. PMID: 39367988

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-NPRL2 Antibody (F-3) has 11 citations in a variety of scientific publications.

Primary antibodies like Anti-NPRL2 Antibody (F-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

NPRL2 Antibody (F-3) Specifications

Alternate Product Name: NPRL2

Product Clone Name: F-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 1-300

Epitope Target: NPRL2

Epitope Species: human

Product Reactivity/Detection: NPRL2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: canine and porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 11 citations in various scientific publications

Available Conjugates/Forms for NPRL2 Antibody (F-3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin