nov Antibody (D-9): sc-136967

nov Antibody (D-9) is a mouse monoclonal IgG1 kappa light chain antibody that detects nov protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). anti-nov antibody (D-9) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. nov protein, also known as nephroblastoma overexpressed, CCN3, or NOVH, plays a crucial role in regulating cellular processes, particularly in tissues where calcium is a significant factor, such as the adrenal gland, central nervous system, bone and cartilage, heart muscle, and kidney. nov protein functions as a negative regulator of cell growth, providing a counterbalance to the positive regulatory effects of other members of the CCN family, such as Cyr61 and CTGF. Aberrant expression of nov has been linked to tumor development, highlighting nov protein′s potential as a biomarker for certain cancers. nov protein interacts with the Notch1 extracellular domain, inhibiting myoblast differentiation through the Notch signaling pathway, which is vital for muscle development and regeneration. The gene encoding nov is located on human chromosome 8q24.12 and was initially identified due to nov protein′s overexpression in virally induced nephroblastoma, making nov a significant focus of research in cancer biology and developmental processes.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

nov Antibody (D-9) References:

1. Nuclear localisation of NOVH protein: a potential role for NOV in the regulation of gene expression.  |  Perbal, B. 1999. Mol Pathol. 52: 84-91. PMID: 10474687
2. NOV (nephroblastoma overexpressed) and the CCN family of genes: structural and functional issues.  |  Perbal, B. 2001. Mol Pathol. 54: 57-79. PMID: 11322167
3. Expression of the human NOV gene in first trimester fetal tissues.  |  Kocialkowski, S., et al. 2001. Anat Embryol (Berl). 203: 417-27. PMID: 11453160
4. Immunocytochemical localization of NOVH protein and ultrastructural characteristics of NCI-H295R cells.  |  Thomopoulos, GN., et al. 2001. J Submicrosc Cytol Pathol. 33: 251-60. PMID: 11846094
5. The nephroblastoma overexpressed gene (NOV/ccn3) protein associates with Notch1 extracellular domain and inhibits myoblast differentiation via Notch signaling pathway.  |  Sakamoto, K., et al. 2002. J Biol Chem. 277: 29399-405. PMID: 12050162
6. A role for CCN3 (NOV) in calcium signalling.  |  Li, CL., et al. 2002. Mol Pathol. 55: 250-61. PMID: 12147716
7. The expression of novH in adrenocortical cells is down-regulated by TGFbeta 1 through c-Jun in a Smad-independent manner.  |  Lafont, J., et al. 2002. J Biol Chem. 277: 41220-9. PMID: 12149257
8. Nephroblastoma overexpressed (Nov) inhibits osteoblastogenesis and causes osteopenia.  |  Rydziel, S., et al. 2007. J Biol Chem. 282: 19762-72. PMID: 17500060
9. Nephroblastoma overexpressed/cysteine-rich protein 61/connective tissue growth factor/nephroblastoma overexpressed gene-3 (NOV/CCN3), a selective adrenocortical cell proapoptotic factor, is down-regulated in childhood adrenocortical tumors.  |  Doghman, M., et al. 2007. J Clin Endocrinol Metab. 92: 3253-60. PMID: 17566092
10. Clinical Significance of Expression of Nephroblastoma Overexpressed (NOV) in Patients with Colorectal Cancer.  |  Ueda, M., et al. 2015. Anticancer Res. 35: 6591-7. PMID: 26637874
11. The Association of Nephroblastoma Overexpressed (NOV) and Endothelial Progenitor Cells with Oxidative Stress in Obstructive Sleep Apnea.  |  Fakhouri, EW., et al. 2021. Oxid Med Cell Longev. 2021: 7138800. PMID: 34868456
12. Nephroblastoma overexpressed protein (NOV) enhances 5-Fu-mediated inhibitory effect of colorectal cancer cell proliferation via JNK/AP-1/caspase-8/caspase-3 pathway.  |  Wang, D., et al. 2021. Discov Oncol. 12: 10. PMID: 35201461

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-nov Antibody (D-9) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-nov Antibody (D-9) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

nov Antibody (D-9) Specifications

Alternate Product Name: nov

Product Clone Name: D-9

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 48-118 deletion aa 104

Epitope Target: nov

Epitope Species: human

Product Reactivity/Detection: nov

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for nov Antibody (D-9): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin