Notch1 Antibody (4i50): sc-71719
- Notch1 Antibody (4i50) is a Syrian hamster monoclonal IgG, cited in 4 publications, provided at 200 µg/ml
- raised against residues 1299-1492 (region EGF repeat 33 through LNG repeat 1) of Notch 1 of mouse origin
- recommended for detection of Notch 1 of mouse and rat origin by WB, IP and IF
- See Notch1 (A-8): sc-376403 for Notch1 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for Notch1 Antibody (4i50). This work is in progress.
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Notch 1 Antibody (4i50) is a hamster monoclonal IgG antibody specifically developed to detect Notch1, also known as Neurogenic locus notch homolog protein 1, in mouse and rat samples via western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). Notch1 monoclonal antibody (4i50) targets an epitope encompassing residues 1299-1492, covering the region from epidermal growth factor (EGF) repeat 33 through the first Lin12/Notch repeat (LNR) of the Notch1 protein, ensuring precise detection. Notch1 is a single-pass transmembrane receptor characterized by an extracellular domain with multiple EGF-like repeats, a negative regulatory region (NRR) containing LNR modules, and an intracellular domain with transcriptional activation capabilities. The structural integrity of these domains is crucial for Notch1′s role in cell fate determination, differentiation, and developmental signaling pathways. Upon ligand binding, conformational changes in the NRR expose cleavage sites, leading to the release of the intracellular domain, which translocates to the nucleus to regulate gene expression. Dysregulation of Notch1 signaling has been implicated in various cancers and developmental disorders, underscoring the importance of structural components in maintaining proper function. Anti-Notch1 antibody (4i50) is available in non-conjugated form as well as multiple conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and various Alexa Fluor® conjugates, catering to diverse experimental needs.
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Notch1 Antibody (4i50) | sc-71719 | 200 µg/ml | $322.00 |