eNOS Antibody (B-5): sc-136977
- eNOS Antibody (B-5) is a mouse monoclonal IgG2b κ, cited in 31 publications, provided at 200 µg/ml
- raised against amino acids 2-160 of NOS3 of human origin
- Anti-eNOS Antibody (B-5) is recommended for detection of NOS3 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-eNOS Antibody (B-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG2b BP-HRP is the preferred secondary detection reagent for eNOS Antibody (B-5) for WB applications. This reagent is now offered in a bundle with eNOS Antibody (B-5) (see ordering information below).
QUICK LINKS
NOS3 Antibody (B-5) is a mouse monoclonal IgG2b kappa light chain antibody that detects NOS3 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). NOS3 antibody (B-5) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. NOS3, also known as endothelial nitric oxide synthase (ecNOS), plays a crucial role in the cardiovascular system by synthesizing nitric oxide (NO), a signaling molecule that regulates vascular tone and blood flow. Proper functioning of NOS3 is vital for maintaining endothelial health and preventing cardiovascular diseases, as NOS3 helps modulate processes such as vasodilation and platelet aggregation. NOS3 activity depends on homodimerization and requires several cofactors, including L-arginine, NADPH, and oxygen, along with essential prosthetic groups like flavin adenine dinucleotide and heme. Understanding NOS3 structure and function is important for developing therapeutic strategies aimed at enhancing NOS3 activity in conditions characterized by impaired nitric oxide production, such as hypertension and atherosclerosis.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
eNOS Antibody (B-5) | sc-136977 | 200 µg/ml | $322.00 | |||
eNOS Antibody (B-5): m-IgG2b BP-HRP Bundle | sc-548667 | 200 µg Ab; 10 µg BP | $361.00 | |||
eNOS Antibody (B-5) AC | sc-136977 AC | 500 µg/ml, 25% agarose | $424.00 | |||
eNOS Antibody (B-5) HRP | sc-136977 HRP | 200 µg/ml | $322.00 | |||
eNOS Antibody (B-5) FITC | sc-136977 FITC | 200 µg/ml | $336.00 | |||
eNOS Antibody (B-5) PE | sc-136977 PE | 200 µg/ml | $349.00 | |||
eNOS Antibody (B-5) Alexa Fluor® 488 | sc-136977 AF488 | 200 µg/ml | $364.00 | |||
eNOS Antibody (B-5) Alexa Fluor® 546 | sc-136977 AF546 | 200 µg/ml | $364.00 | |||
eNOS Antibody (B-5) Alexa Fluor® 594 | sc-136977 AF594 | 200 µg/ml | $364.00 | |||
eNOS Antibody (B-5) Alexa Fluor® 647 | sc-136977 AF647 | 200 µg/ml | $364.00 | |||
eNOS Antibody (B-5) Alexa Fluor® 680 | sc-136977 AF680 | 200 µg/ml | $364.00 | |||
eNOS Antibody (B-5) Alexa Fluor® 790 | sc-136977 AF790 | 200 µg/ml | $364.00 |