Nore1 Antibody (10F10): sc-80017
- Nore1 Antibody (10F10) is a mouse monoclonal IgG1 κ Nore1 antibody provided at 200 µg/ml
- raised against amino acids 119-414 of Nore1 of human origin
- Nore1 Antibody (10F10) is recommended for detection of an epitope corresponding to amino acids 119-190 of Nore1 of human origin by WB, IP and IF
- Anti-Nore1 Antibody (10F10) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for Nore1 Antibody (10F10) for WB applications. These reagents are now offered in bundles with Nore1 Antibody (10F10) (see ordering information below).
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Nore1 Antibody (10F10) is a mouse monoclonal IgG1 kappa light chain antibody that detects the Nore1 protein of human origin by western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). Nore1 (10F10) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Nore1, also known as RASSF5 (ras association domain-containing family protein 5), RAPL, or Maxp1, is a 418 amino acid protein that exists as four alternatively spliced isoforms, which localize to the cytoplasm, growing microtubules, and the perinuclear region of unstimulated primary T cells. This protein plays a crucial role as a tumor suppressor, significantly contributing to lymphocyte adhesion, polarization, apoptotic induction, and the directional movement of endothelial cells during wound healing. Notably, Nore1 contains a SARAH domain, a phorbol-ester/DAG-type zinc finger, and a Ras-associating domain, which facilitate interaction with several Ras-like GTPases. These interactions are vital for regulating cellular growth and preventing tumor transformation, underscoring Nore1′s importance in maintaining cellular homeostasis and potential implications in cancer biology.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Nore1 Antibody (10F10) References:
- RASSF3 and NORE1: identification and cloning of two human homologues of the putative tumor suppressor gene RASSF1. | Tommasi, S., et al. 2002. Oncogene. 21: 2713-20. PMID: 11965544
- Chromosome mapping and sequence variation of the murine Ras effector gene Nore1. | Yao, R., et al. 2001. Cytogenet Cell Genet. 95: 126-8. PMID: 11978988
- RAPL, a Rap1-binding molecule that mediates Rap1-induced adhesion through spatial regulation of LFA-1. | Katagiri, K., et al. 2003. Nat Immunol. 4: 741-8. PMID: 12845325
- Regulation of the MST1 kinase by autophosphorylation, by the growth inhibitory proteins, RASSF1 and NORE1, and by Ras. | Praskova, M., et al. 2004. Biochem J. 381: 453-62. PMID: 15109305
- Crucial functions of the Rap1 effector molecule RAPL in lymphocyte and dendritic cell trafficking. | Katagiri, K., et al. 2004. Nat Immunol. 5: 1045-51. PMID: 15361866
- Characterization of interactions of adapter protein RAPL/Nore1B with RAP GTPases and their role in T cell migration. | Miertzschke, M., et al. 2007. J Biol Chem. 282: 30629-42. PMID: 17716979
- Assessment of NORE1A as a putative tumor suppressor in human neuroblastoma. | Geli, J., et al. 2008. Int J Cancer. 123: 389-394. PMID: 18452173
- Novel type of Ras effector interaction established between tumour suppressor NORE1A and Ras switch II. | Stieglitz, B., et al. 2008. EMBO J. 27: 1995-2005. PMID: 18596699
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Nore1 Antibody (10F10) | sc-80017 | 200 µg/ml | $316.00 | |||
Nore1 Antibody (10F10): m-IgG Fc BP-HRP Bundle | sc-527050 | 200 µg Ab; 10 µg BP | $354.00 | |||
Nore1 Antibody (10F10): m-IgG1 BP-HRP Bundle | sc-532423 | 200 µg Ab; 20 µg BP | $354.00 | |||
Nore1 Antibody (10F10) AC | sc-80017 AC | 500 µg/ml, 25% agarose | $416.00 | |||
Nore1 Antibody (10F10) HRP | sc-80017 HRP | 200 µg/ml | $316.00 | |||
Nore1 Antibody (10F10) FITC | sc-80017 FITC | 200 µg/ml | $330.00 | |||
Nore1 Antibody (10F10) PE | sc-80017 PE | 200 µg/ml | $343.00 | |||
Nore1 Antibody (10F10) Alexa Fluor® 488 | sc-80017 AF488 | 200 µg/ml | $357.00 | |||
Nore1 Antibody (10F10) Alexa Fluor® 546 | sc-80017 AF546 | 200 µg/ml | $357.00 | |||
Nore1 Antibody (10F10) Alexa Fluor® 594 | sc-80017 AF594 | 200 µg/ml | $357.00 | |||
Nore1 Antibody (10F10) Alexa Fluor® 647 | sc-80017 AF647 | 200 µg/ml | $357.00 | |||
Nore1 Antibody (10F10) Alexa Fluor® 680 | sc-80017 AF680 | 200 µg/ml | $357.00 | |||
Nore1 Antibody (10F10) Alexa Fluor® 790 | sc-80017 AF790 | 200 µg/ml | $357.00 |