Nop25 Antibody (E-2): sc-374257
- Nop25 Antibody (E-2) is a mouse monoclonal IgG1 κ Nop25 antibody, cited in 2 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 141-171 within an internal region of Nop25 of human origin
- Nop25 Antibody (E-2) is recommended for detection of Nop25 of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including and equine, bovine and porcine
- Anti-Nop25 Antibody (E-2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for Nop25 Antibody (E-2) for WB applications. These reagents are now offered in bundles with Nop25 Antibody (E-2) (see ordering information below).
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Nop25 Antibody (E-2) is a mouse monoclonal IgG1 kappa light chain antibody that detects Nop25 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Nop25 (E-2) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Nop25, also known as NOL12 (nucleolar protein 12), consists of 213 amino acids and is primarily localized in the nucleolus, where Nop25 is believed to interact with 28S rRNA. This interaction is crucial as Nop25 plays a significant role in the processing of ribosomal RNA molecules and the subsequent assembly and maturation of ribosomes, which are essential for protein synthesis in all living cells. The gene encoding Nop25 is located on human chromosome 22, a region that contains over 500 genes and is associated with various genetic disorders, including Phelan-McDermid syndrome, Neurofibromatosis type 2, autism, and schizophrenia. Furthermore, chromosomal translocations involving chromosome 22 can lead to the formation of the Philadelphia Chromosome, resulting in the production of the BCR-Abl fusion protein, a potent activator of cell proliferation implicated in several types of leukemia.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Nop25 Antibody (E-2) References:
- Chromosome 22 workshop report. | Schwab, SG. and Wildenauer, DB. 1999. Am J Med Genet. 88: 276-8. PMID: 10374745
- Disease genes and chromosomes: disease maps of the human genome. Chromosome 22. | Gilbert, F. 1998. Genet Test. 2: 89-97. PMID: 10464604
- Constitutional rearrangements of chromosome 22 as a cause of neurofibromatosis 2. | Tsilchorozidou, T., et al. 2004. J Med Genet. 41: 529-34. PMID: 15235024
- Molecular cloning and characterization of Nop25, a novel nucleolar RNA binding protein, highly conserved in vertebrate species. | Suzuki, S., et al. 2006. Exp Cell Res. 312: 1031-41. PMID: 16430885
- Mapping a nucleolar targeting sequence of an RNA binding nucleolar protein, Nop25. | Fujiwara, T., et al. 2006. Exp Cell Res. 312: 1703-12. PMID: 16515785
- Tbx1 haploinsufficiency is linked to behavioral disorders in mice and humans: implications for 22q11 deletion syndrome. | Paylor, R., et al. 2006. Proc Natl Acad Sci U S A. 103: 7729-34. PMID: 16684884
- Analyses of the associations between the genes of 22q11 deletion syndrome and schizophrenia. | Arinami, T. 2006. J Hum Genet. 51: 1037-1045. PMID: 16969581
- BCR and its mutants, the reciprocal t(9;22)-associated ABL/BCR fusion proteins, differentially regulate the cytoskeleton and cell motility. | Zheng, X., et al. 2006. BMC Cancer. 6: 262. PMID: 17090304
- Nucleolar protein Nop25 is involved in nucleolar architecture. | Suzuki, S., et al. 2007. Biochem Biophys Res Commun. 358: 1114-9. PMID: 17524363
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Nop25 Antibody (E-2) | sc-374257 | 200 µg/ml | $322.00 | |||
Nop25 Antibody (E-2): m-IgG Fc BP-HRP Bundle | sc-527447 | 200 µg Ab; 10 µg BP | $361.00 | |||
Nop25 Antibody (E-2): m-IgG1 BP-HRP Bundle | sc-532820 | 200 µg Ab; 20 µg BP | $361.00 | |||
Nop25 Antibody (E-2) AC | sc-374257 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Nop25 Antibody (E-2) HRP | sc-374257 HRP | 200 µg/ml | $322.00 | |||
Nop25 Antibody (E-2) FITC | sc-374257 FITC | 200 µg/ml | $336.00 | |||
Nop25 Antibody (E-2) PE | sc-374257 PE | 200 µg/ml | $349.00 | |||
Nop25 Antibody (E-2) Alexa Fluor® 488 | sc-374257 AF488 | 200 µg/ml | $364.00 | |||
Nop25 Antibody (E-2) Alexa Fluor® 546 | sc-374257 AF546 | 200 µg/ml | $364.00 | |||
Nop25 Antibody (E-2) Alexa Fluor® 594 | sc-374257 AF594 | 200 µg/ml | $364.00 | |||
Nop25 Antibody (E-2) Alexa Fluor® 647 | sc-374257 AF647 | 200 µg/ml | $364.00 | |||
Nop25 Antibody (E-2) Alexa Fluor® 680 | sc-374257 AF680 | 200 µg/ml | $364.00 | |||
Nop25 Antibody (E-2) Alexa Fluor® 790 | sc-374257 AF790 | 200 µg/ml | $364.00 | |||
Nop25 (E-2) Neutralizing Peptide | sc-374257 P | 100 µg/0.5 ml | $69.00 |