Nop14 Double Nickase Plasmid (h): sc-408567-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Nop14 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Nop14 Double Nickase Plasmid (h) and Nop14 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting NOP14. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Nop14 Antibody (B-5): sc-398763
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Nop14 Double Nickase Plasmid (h) | sc-408567-NIC | 20 µg | $410.00 |
Human NOP14 encodes Nop14, a nucleolar protein required for ribosome biogenesis, particularly maturation of the 40S small ribosomal subunit through pre-rRNA processing and assembly of preribosomal particles. By supporting efficient ribosome production, Nop14 influences cellular growth control, cell cycle progression, and proteostasis pathways that are tightly coupled to nucleolar function and stress responses. Altered regulation of ribosome biogenesis factors, including NOP14, has been studied in the context of dysregulated proliferation and genomic instability, making it relevant for mechanistic investigations in cancer biology and other disorders linked to nucleolar dysfunction. NOP14 is also used as a node to examine how perturbations in ribosome assembly remodel global translation programs and downstream signaling networks.
Nop14 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the NOP14 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within NOP14. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt NOP14 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of NOP14-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.