Nop132 Antibody (D-7): sc-390011

Nop132 Antibody (D-7) is a mouse monoclonal IgM antibody that detects Nop132 protein of human origin and NOL8 protein of mouse and rat origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-Nop132 antibody (D-7) is available as the non-conjugated form. Nop132, also known as NOL8 (nucleolar protein 8), is a 1,167 amino acid nuclear protein characterized by one RNA recognition motif (RRM) domain and exists in four alternatively spliced isoforms. Nop132 plays a crucial role in regulating post-transcriptional gene expression and ribosome biogenesis, particularly in diffuse-type gastric cancer, where Nop132 is essential for cancer cell survival. While Nop132 shows low expression in skeletal muscle, Nop132 expression significantly increases in diffuse-type gastric cancers, highlighting potential use as a biomarker for this malignancy. Nop132 interacts with several key proteins, including NIP7 and GTP-bound forms of Rag A/B, Rag C, and Rag D, which are involved in cellular signaling pathways that regulate nutrient sensing and mTOR signaling. The Nop132 gene spans approximately 28,237 bases on human chromosome 9q22.31, a region housing over 900 genes and implicated in various genetic disorders, including hereditary hemorrhagic telangiectasia and familial dysautonomia. Chromosome 9 contains the largest cluster of interferon family genes, underscoring importance in immune response regulation.

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Nop132 Antibody (D-7) References:

1. Novel G proteins, Rag C and Rag D, interact with GTP-binding proteins, Rag A and Rag B.  |  Sekiguchi, T., et al. 2001. J Biol Chem. 276: 7246-57. PMID: 11073942
2. A novel human nucleolar protein, Nop132, binds to the G proteins, RRAG A/C/D.  |  Sekiguchi, T., et al. 2004. J Biol Chem. 279: 8343-50. PMID: 14660641
3. Identification of NOL8, a nucleolar protein containing an RNA recognition motif (RRM), which was overexpressed in diffuse-type gastric cancer.  |  Jinawath, N., et al. 2004. Cancer Sci. 95: 430-5. PMID: 15132771
4. Pulmonary vascular manifestations of hereditary hemorrhagic telangiectasia (rendu-osler disease).  |  Cottin, V., et al. 2007. Respiration. 74: 361-78. PMID: 17641482
5. Atypical BCR-ABL mRNA transcripts in adult acute lymphoblastic leukemia.  |  Burmeister, T., et al. 2007. Haematologica. 92: 1699-702. PMID: 18055996
6. Kinetin in familial dysautonomia carriers: implications for a new therapeutic strategy targeting mRNA splicing.  |  Gold-von Simson, G., et al. 2009. Pediatr Res. 65: 341-6. PMID: 19033881
7. Organization of the amplified type I interferon gene cluster and associated chromosome regions in the interphase nucleus of human osteosarcoma cells.  |  Zeitz, MJ., et al. 2009. Chromosome Res. 17: 305-19. PMID: 19283497
8. Neuroimaging supports central pathology in familial dysautonomia.  |  Axelrod, FB., et al. 2010. J Neurol. 257: 198-206. PMID: 19705052

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Nop132 Antibody (D-7) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-Nop132 Antibody (D-7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Nop132 Antibody (D-7) Specifications

Alternate Product Name: Nop132

Product Clone Name: D-7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgM kappa light chain

Amino Acids: 181-215

Epitope Target: Nop132

Epitope Species: human

Product Reactivity/Detection: Nop132 of human origin and NOL8 of mouse and rat origin

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: bovine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgM kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for Nop132 Antibody (D-7): P: Blocking Peptide