Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Nodal CRISPR/Cas9 KO Plasmid (h): sc-401439
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Nodal CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Nodal genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Nodal Antibody (G-10): sc-373910
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Nodal CRISPR/Cas9 KO Plasmid (h) | sc-401439 | 20 µg | $397.00 |
Overview
NODAL encodes Nodal, a secreted TGF-β superfamily morphogen that signals primarily through Activin receptors and SMAD2/3 to regulate mesendoderm specification, left–right patterning, and maintenance of pluripotency programs during early development. In human cells, Nodal integrates with feedback regulators such as LEFTY and with crosstalk to WNT and FGF pathways to shape lineage decisions, epithelial–mesenchymal transitions, and transcriptional networks controlling stem cell states. Aberrant NODAL expression has been reported in multiple tumor contexts and is frequently studied for its association with invasive phenotypes, cellular plasticity, and resistance-associated signaling circuitry. As a pathway node, Nodal is commonly interrogated in developmental biology, cancer biology, and stem cell differentiation models.
Nodal CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the NODAL gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the NODAL together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the NODAL open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Nodal protein expression.
This CRISPR knockout system enables efficient generation of NODAL-deficient cell models for investigation of Nodal signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting NODAL exon(s) critical for Nodal function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple NODAL genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Nodal CRISPR/Cas9 KO Plasmid (h) and Nodal CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the NODAL locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Nodal HDR Plasmid (h) and Nodal HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by NODAL homology arms to support homology-directed repair at defined NODAL target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.