NOD3 Antibody (E-4): sc-398947

NOD3 Antibody (E-4) is a mouse monoclonal IgG1 kappa light chain antibody that detects NOD3 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). NOD3 (E-4) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. NOD3 protein, also known as NLRC3, is a 1,065 amino acid protein that plays a crucial role in the immune response by modulating T-cell activation. NOD3 is primarily located in the cytoplasm and contains a NACHT domain along with 17 leucine-rich repeats (LRRs), which are essential for protein-protein interactions and signaling pathways. The structure of NOD3, particularly its LRRs, allows for formation of a hydrophobic α/β horseshoe fold, which is vital for function in recognizing and responding to intracellular pathogens. NOD3 is expressed in peripheral blood mononuclear cells and is involved in transcriptional control events related to T-cell activation, highlighting importance in adaptive immunity. Multiple isoforms of NOD3 arise from alternative splicing, further diversifying functional roles in immune regulation.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

NOD3 Antibody (E-4) References:

1. The leucine-rich repeat as a protein recognition motif.  |  Kobe, B. and Kajava, AV. 2001. Curr Opin Struct Biol. 11: 725-32. PMID: 11751054
2. NODs: intracellular proteins involved in inflammation and apoptosis.  |  Inohara, N. and Nuñez, G. 2003. Nat Rev Immunol. 3: 371-82. PMID: 12766759
3. CATERPILLER 16.2 (CLR16.2), a novel NBD/LRR family member that negatively regulates T cell function.  |  Conti, BJ., et al. 2005. J Biol Chem. 280: 18375-85. PMID: 15705585
4. Structural analysis of leucine-rich-repeat variants in proteins associated with human diseases.  |  Matsushima, N., et al. 2005. Cell Mol Life Sci. 62: 2771-91. PMID: 16231091
5. Evolutionary relationships of vertebrate NACHT domain-containing proteins.  |  Hughes, AL. 2006. Immunogenetics. 58: 785-91. PMID: 17006665
6. Monarch-1/PYPAF7 and other CATERPILLER (CLR, NOD, NLR) proteins with negative regulatory functions.  |  Lich, JD. and Ting, JP. 2007. Microbes Infect. 9: 672-6. PMID: 17418609
7. Downregulation of immune signaling genes in patients with large surface burn injury.  |  Moore, CB., et al. 2007. J Burn Care Res. 28: 879-87. PMID: 17925653
8. Constitutive and UV-B modulated transcription of Nod-like receptors and their functional partners in human corneal epithelial cells.  |  Benko, S., et al. 2008. Mol Vis. 14: 1575-83. PMID: 18769647
9. Early action of pea symbiotic gene NOD3 is confirmed by adventitious root phenotype.  |  Novák, K. 2010. Plant Sci. 179: 472-8. PMID: 21802605
10. NLRC3 is an inhibitory sensor of PI3K-mTOR pathways in cancer.  |  Karki, R., et al. 2016. Nature. 540: 583-587. PMID: 27951586
11. Proteins with leucine-rich repeats.  |  Kobe, B. and Deisenhofer, J. 1995. Curr Opin Struct Biol. 5: 409-16. PMID: 7583641
12. The leucine-rich repeat: a versatile binding motif.  |  Kobe, B. and Deisenhofer, J. 1994. Trends Biochem Sci. 19: 415-21. PMID: 7817399

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-NOD3 Antibody (E-4) has 5 citations in a variety of scientific publications.

Primary antibodies like Anti-NOD3 Antibody (E-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

NOD3 Antibody (E-4) Specifications

Alternate Product Name: NOD3

Product Clone Name: E-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1041-1061

Epitope Target: NOD3

Epitope Species: human

Product Reactivity/Detection: NOD3

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 5 citations in various scientific publications

Available Conjugates/Forms for NOD3 Antibody (E-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin