Nocturnin Lentiviral Activation Particles (h): sc-405350-LAC

Human NOCT encodes Nocturnin, a circadian-regulated CCR4-like deadenylase that modulates mRNA poly(A) tail length to control transcript stability and translation in a time-of-day–dependent manner. Nocturnin integrates signals from the circadian clock with metabolic programs, influencing lipid handling, mitochondrial function, and nutrient-responsive gene expression across diverse cell types. Through post-transcriptional regulation, it shapes pathways linked to energy homeostasis, inflammatory signaling, and cellular stress adaptation. Dysregulated NOCT expression or activity has been associated with metabolic phenotypes and altered growth control in disease-relevant contexts, motivating mechanistic studies of how rhythmic RNA turnover impacts pathology.

Nocturnin Lentiviral Activation Particles (h) address this need by packaging the complete synergistic activation mediator (SAM) transcriptional activation system into transduction-ready, high-titer lentiviral particles, enabling efficient NOCT upregulation across a broader range of human cell types.

Nocturnin Lentiviral Activation Particles (h) deliver all functional components of the synergistic activation mediator (SAM) system via lentiviral transduction. The system comprises three particle preparations co-transduced into target cells: one encoding catalytically inactive dCas9 (D10A and N863A mutations) fused to the VP64 transactivation domain with a blasticidin resistance gene; one encoding the MS2-p65-HSF1 fusion protein with a hygromycin resistance gene; and one encoding a target-specific 20 nt sgRNA fused to two MS2 RNA aptamers with a puromycin resistance gene. Following lentiviral transduction and genomic integration of the expression cassettes, the SAM components are stably expressed and assemble at the target locus within the proximal promoter region upstream of the NOCT transcriptional start site, where VP64, p65, and HSF1 act cooperatively to recruit endogenous transcriptional machinery and drive sustained upregulation of endogenous Nocturnin expression. The use of nuclease-inactive dCas9 avoids the introduction of double-strand DNA breaks and preserves the native NOCT genomic locus and regulatory architecture.

The lentiviral format offers several practical advantages: stable genomic integration supports heritable activation across cell divisions; high-titer particle preparations eliminate the need for in-house viral production; and compatibility with primary, non-dividing, and transfection-resistant cell types expands experimental accessibility. Successful transduction can be confirmed and enriched through triple antibiotic selection using puromycin, hygromycin, and blasticidin.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.