NMUR2 CRISPR/Cas9 KO Plasmid (h): sc-404637
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- NMUR2 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the NMUR2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
NMUR2 CRISPR/Cas9 KO Plasmid (h) | sc-404637 | 20 µg | $397.00 |
Overview
Neuromedin U receptor 2 (NMUR2) is a G protein–coupled receptor predominantly expressed in the central nervous system that mediates signaling by the neuropeptide neuromedin U. NMUR2 activation primarily engages Gq/11-dependent pathways to elevate intracellular calcium and modulate MAPK and other second-messenger cascades that influence neuronal excitability and neuroendocrine outputs. Through these signaling networks, NMUR2 contributes to regulation of energy balance, appetite, stress responsiveness, and circadian-linked behaviors. Altered NMUR2 signaling has been investigated in contexts of metabolic dysregulation and neurobehavioral phenotypes, supporting its utility as a target in mechanistic studies of brain–metabolism communication.
NMUR2 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the NMUR2 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the NMUR2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the NMUR2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish NMUR2 protein expression.
This CRISPR knockout system enables efficient generation of NMUR2-deficient cell models for investigation of NMUR2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting NMUR2 exon(s) critical for NMUR2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple NMUR2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by NMUR2 CRISPR/Cas9 KO Plasmid (h) and NMUR2 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the NMUR2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by NMUR2 HDR Plasmid (h) and NMUR2 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by NMUR2 homology arms to support homology-directed repair at defined NMUR2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.