NMDAζ1 Double Nickase Plasmid (h): sc-400593-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- NMDAζ1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- NMDAζ1 Double Nickase Plasmid (h) and NMDAζ1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting GRIN1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: NMDAζ1 Antibody (E-2): sc-518043
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
NMDAζ1 Double Nickase Plasmid (h) | sc-400593-NIC | 20 µg | $410.00 | |||
NMDAζ1 Double Nickase Plasmid (h2) | sc-400593-NIC-2 | 20 µg | $410.00 |
GRIN1 encodes the human NMDA receptor ζ1 (GluN1) subunit, an essential component of NMDA-type ionotropic glutamate receptors that assemble as heterotetrameric channels to mediate Ca²⁺-permeable excitatory neurotransmission. NMDA receptor signaling regulates synaptic plasticity, learning and memory, and activity-dependent gene expression through pathways involving CaMKII/CREB and downstream transcriptional programs. GRIN1-dependent receptor function also intersects with neuronal development processes such as synaptogenesis and dendritic remodeling, and it contributes to excitotoxic stress responses under conditions of excessive glutamatergic stimulation. Genetic variation or dysregulated NMDA receptor signaling has been associated with neurodevelopmental and neuropsychiatric phenotypes, supporting mechanistic studies of receptor function in disease-relevant cellular models.
NMDAζ1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the GRIN1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within GRIN1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt GRIN1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of GRIN1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.