NMDAε4 Double Nickase Plasmid (h): sc-401683-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- NMDAε4 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- NMDAε4 Double Nickase Plasmid (h) and NMDAε4 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting GRIN2D. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: NMDAε4 Antibody (G-10): sc-17822
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
NMDAε4 Double Nickase Plasmid (h) | sc-401683-NIC | 20 µg | $410.00 | |||
NMDAε4 Double Nickase Plasmid (h2) | sc-401683-NIC-2 | 20 µg | $410.00 |
GRIN2D encodes the human NMDA receptor subunit NMDAε4 (GluN2D), a ligand-gated ion channel component that assembles with GluN1 to form functional N-methyl-D-aspartate receptors. NMDAε4 contributes to glutamatergic synaptic transmission by regulating Ca²⁺ influx, receptor gating kinetics, and downstream activity-dependent signaling that shapes neuronal development and circuit excitability. Through coupling to calcium-dependent pathways, it influences synaptic plasticity, transcriptional programs, and excitotoxic stress responses in the central nervous system. Variation or dysregulated expression of GRIN2D has been linked in the literature to neurodevelopmental and seizure-related phenotypes, supporting its use in mechanistic studies of glutamate signaling and neuronal network function.
NMDAε4 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the GRIN2D locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within GRIN2D. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt GRIN2D function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of GRIN2D-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.