NMDAε2 Double Nickase Plasmid (h): sc-400654-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- NMDAε2 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- NMDAε2 Double Nickase Plasmid (h) and NMDAε2 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting GRIN2B. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: NMDAε2 Antibody (A-8): sc-365597
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
NMDAε2 Double Nickase Plasmid (h) | sc-400654-NIC | 20 µg | $410.00 | |||
NMDAε2 Double Nickase Plasmid (h2) | sc-400654-NIC-2 | 20 µg | $410.00 |
GRIN2B encodes the NMDAε2 (GluN2B) subunit of N-methyl-D-aspartate receptors, ligand-gated ion channels that regulate Ca²⁺ influx during excitatory neurotransmission. NMDAε2-containing receptors shape synaptic plasticity, learning, and memory by coupling neuronal activity to downstream signaling pathways such as CaMKII/CREB-dependent transcription, MAPK/ERK signaling, and activity-dependent cytoskeletal remodeling. GRIN2B function is closely linked to glutamatergic synapse development and excitotoxic stress responses, integrating signals that influence dendritic spine maturation and circuit refinement. Genetic variation and dysregulation of GRIN2B have been associated with neurodevelopmental and neuropsychiatric phenotypes, supporting its use in mechanistic studies of synaptic signaling and neuronal network function.
NMDAε2 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the GRIN2B locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within GRIN2B. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt GRIN2B function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of GRIN2B-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.