NMDAε1 Double Nickase Plasmid (m): sc-420689-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- NMDAε1 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- NMDAε1 Double Nickase Plasmid (m) and NMDAε1 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Grin2a. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: NMDAε1 Antibody (E-4): sc-515148
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
NMDAε1 Double Nickase Plasmid (m) | sc-420689-NIC | 20 µg | $410.00 | |||
NMDAε1 Double Nickase Plasmid (m2) | sc-420689-NIC-2 | 20 µg | $410.00 |
Grin2a encodes the NMDA receptor subunit NMDA ε1 (NR2A), a glutamate-gated ion channel component that shapes synaptic transmission through activity-dependent Ca²⁺ influx. NR2A-containing NMDA receptors are central to excitatory neurotransmission, synaptic plasticity, and neuronal circuit refinement, coupling receptor activation to downstream CaMK/CREB signaling, MAPK/ERK pathways, and transcriptional programs that regulate learning and memory. In mouse brain, GRIN2A helps tune receptor kinetics and synaptic maturation, influencing the balance of excitation and inhibition. Dysregulated GRIN2A/NMDA receptor signaling has been linked to neurodevelopmental and seizure-related phenotypes in experimental models, supporting its use in mechanistic studies of cognition and network excitability.
NMDAε1 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Grin2a locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Grin2a. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Grin2a function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Grin2a-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.