NMDAε1 Double Nickase Plasmid (h): sc-400963-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- NMDAε1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- NMDAε1 Double Nickase Plasmid (h) and NMDAε1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting GRIN2A. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: NMDAε1 Antibody (E-4): sc-515148
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
NMDAε1 Double Nickase Plasmid (h) | sc-400963-NIC | 20 µg | $410.00 | |||
NMDAε1 Double Nickase Plasmid (h2) | sc-400963-NIC-2 | 20 µg | $410.00 |
GRIN2A encodes the human NMDA receptor subunit NMDAε1 (GluN2A), a glutamate-gated ion channel component that assembles with GluN1 to form receptors mediating Ca²⁺ influx during excitatory neurotransmission. NMDAε1-containing receptors regulate synaptic plasticity, including long-term potentiation, and couple neuronal activity to downstream signaling pathways such as CaMKII/CREB, MAPK/ERK, and activity-dependent gene expression programs. Through these processes, GRIN2A influences circuit development, learning and memory, and excitatory–inhibitory balance. Genetic variation or dysregulated expression of GRIN2A has been linked to neurodevelopmental and epilepsy-related phenotypes, making it a key target for mechanistic studies of synaptic signaling and network excitability.
NMDAε1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the GRIN2A locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within GRIN2A. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt GRIN2A function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of GRIN2A-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.