NLRC5 Double Nickase Plasmid (m): sc-436677-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- NLRC5 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- NLRC5 Double Nickase Plasmid (m) and NLRC5 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Nlrc5. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: NLRC5 Antibody (B-10): sc-515668
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
NLRC5 Double Nickase Plasmid (m) | sc-436677-NIC | 20 µg | $410.00 | |||
NLRC5 Double Nickase Plasmid (m2) | sc-436677-NIC-2 | 20 µg | $410.00 |
Nlrc5 encodes NLRC5, an NLR family cytosolic sensor that functions as a key transcriptional regulator of MHC class I antigen presentation by promoting expression of H2-K/H2-D, β2-microglobulin, and antigen-processing components. NLRC5 intersects with innate immune signaling networks downstream of interferon pathways and can modulate inflammatory transcriptional programs, linking pathogen sensing to adaptive immune priming. In mouse systems, altered Nlrc5 activity has been associated with changes in immune surveillance, susceptibility to infection, and regulation of tumor–immune interactions. These features make NLRC5 a useful node for dissecting antigen presentation dynamics, immune evasion mechanisms, and interferon-driven gene expression in relevant cellular models.
NLRC5 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Nlrc5 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Nlrc5. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Nlrc5 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Nlrc5-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.