NLP CRISPR/Cas9 KO Plasmid (h): sc-406794
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- NLP CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the NLP genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
NLP CRISPR/Cas9 KO Plasmid (h) | sc-406794 | 20 µg | $397.00 |
Overview
NINL encodes ninein-like protein (NLP), a centrosome- and microtubule-associated factor that supports centrosomal organization, microtubule anchoring, and cytoskeletal dynamics required for intracellular trafficking and cell division. NLP is linked to centrosome maturation and microtubule nucleation/organization processes that shape spindle formation and maintain genome stability during mitosis. Perturbation of centrosome and spindle regulation is relevant to proliferative disorders and chromosomal instability phenotypes, making NINL a useful target for dissecting pathways that couple centrosome function to cell-cycle progression. In human cells, NINL studies also inform mechanisms of microtubule-dependent transport and spatial organization of organelles.
NLP CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the NINL gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the NINL together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the NINL open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish NLP protein expression.
This CRISPR knockout system enables efficient generation of NINL-deficient cell models for investigation of NLP signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting NINL exon(s) critical for NLP function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple NINL genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by NLP CRISPR/Cas9 KO Plasmid (h) and NLP CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the NINL locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by NLP HDR Plasmid (h) and NLP HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by NINL homology arms to support homology-directed repair at defined NINL target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.