Nkx-2.2 CRISPR/Cas9 KO Plasmid (m): sc-421901
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Nkx-2.2 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Nkx-2.2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Nkx-2.2 Antibody (D-4): sc-398951
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Nkx-2.2 CRISPR/Cas9 KO Plasmid (m) | sc-421901 | 20 µg | $397.00 |
Overview
Nkx2-2 encodes the homeobox transcription factor Nkx-2.2, a nuclear regulator essential for embryonic patterning and lineage specification in the developing central nervous system and endoderm-derived tissues. In the ventral neural tube, Nkx-2.2 functions downstream of Sonic hedgehog signaling to establish progenitor domains and drive differentiation programs, coordinating gene regulatory networks that shape neuronal subtype identity. In the pancreas, Nkx-2.2 contributes to endocrine cell development and maturation by controlling transcriptional programs linked to islet lineage allocation and hormone expression. Dysregulation of NKX2-2–dependent transcriptional circuits has been associated with altered neurodevelopmental trajectories and perturbations of endocrine differentiation, supporting its use as a mechanistic node in developmental biology and disease-modeling studies.
Nkx-2.2 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Nkx2-2 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Nkx2-2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Nkx2-2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Nkx-2.2 protein expression.
This CRISPR knockout system enables efficient generation of Nkx2-2-deficient cell models for investigation of Nkx-2.2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Nkx2-2 exon(s) critical for Nkx-2.2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Nkx2-2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Nkx-2.2 CRISPR/Cas9 KO Plasmid (m) and Nkx-2.2 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Nkx2-2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Nkx-2.2 HDR Plasmid (m) and Nkx-2.2 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Nkx2-2 homology arms to support homology-directed repair at defined Nkx2-2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.