NKCC2 Antibody (4H4): sc-293222

NKCC2 Antibody (4H4) is a mouse monoclonal IgG2a kappa light chain antibody that detects NKCC2 protein of human origin by western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). Anti-NKCC2 antibody (4H4) is available as a non-conjugated monoclonal isotype antibody. NKCC2 plays a crucial role in the kidney, specifically in the thick ascending limb of the loop of Henle, where NKCC2 is responsible for active reabsorption of sodium and chloride ions. This function is vital for maintaining electrolyte balance and blood pressure regulation in the body. The gene encoding human NKCC2 is located on chromosome 15q15-q21, and mutations in this gene can lead to Bartter′s syndrome, a genetic disorder characterized by hypokalemic alkalosis and metabolic imbalances. NKCC2 is sensitive to diuretics such as furosemide and bumetanide, which are commonly used in clinical settings to manage conditions like hypertension and edema. Understanding NKCC2 function and regulation is essential for developing targeted therapies for renal disorders and improving patient outcomes.

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NKCC2 Antibody (4H4) References:

1. Deafness and imbalance associated with inactivation of the secretory Na-K-2Cl co-transporter.  |  Delpire, E., et al. 1999. Nat Genet. 22: 192-5. PMID: 10369265
2. Adenylyl cyclase 6 enhances NKCC2 expression and mediates vasopressin-induced phosphorylation of NKCC2 and NCC.  |  Rieg, T., et al. 2013. Am J Pathol. 182: 96-106. PMID: 23123217
3. Diacidic Motifs in the Carboxyl Terminus Are Required for ER Exit and Translocation to the Plasma Membrane of NKCC2.  |  Bakhos-Douaihy, D., et al. 2022. Int J Mol Sci. 23: PMID: 36361553
4. Dysregulation of the WNK4-SPAK/OSR1 pathway has a minor effect on baseline NKCC2 phosphorylation.  |  Maeoka, Y., et al. 2024. Am J Physiol Renal Physiol. 326: F39-F56. PMID: 37881876
5. AUP1 Regulates the Endoplasmic Reticulum-Associated Degradation and Polyubiquitination of NKCC2.  |  Frachon, N., et al. 2024. Cells. 13: PMID: 38474353
6. Update on NKCC2 regulation in the thick ascending limb (TAL) by membrane trafficking, phosphorylation, and protein-protein interactions.  |  Maskey, D., et al. 2024. Front Physiol. 15: 1508806. PMID: 39717823
7. Primary structure, functional expression, and chromosomal localization of the bumetanide-sensitive Na-K-Cl cotransporter in human colon.  |  Payne, JA., et al. 1995. J Biol Chem. 270: 17977-85. PMID: 7629105
8. Molecular cloning and functional expression of the bumetanide-sensitive Na-K-Cl cotransporter.  |  Xu, JC., et al. 1994. Proc Natl Acad Sci U S A. 91: 2201-5. PMID: 8134373
9. Gitelman's variant of Bartter's syndrome, inherited hypokalaemic alkalosis, is caused by mutations in the thiazide-sensitive Na-Cl cotransporter.  |  Simon, DB., et al. 1996. Nat Genet. 12: 24-30. PMID: 8528245
10. Localization of the renal Na-K-Cl cotransporter gene (Slc12a1) on mouse chromosome 2.  |  Quaggin, SE., et al. 1995. Mamm Genome. 6: 557-8. PMID: 8589530
11. Molecular cloning, expression pattern, and chromosomal localization of the human Na-Cl thiazide-sensitive cotransporter (SLC12A3).  |  Mastroianni, N., et al. 1996. Genomics. 35: 486-93. PMID: 8812482
12. Novel molecular variants of the Na-Cl cotransporter gene are responsible for Gitelman syndrome.  |  Mastroianni, N., et al. 1996. Am J Hum Genet. 59: 1019-26. PMID: 8900229

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-NKCC2 Antibody (4H4) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-NKCC2 Antibody (4H4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

NKCC2 Antibody (4H4) Specifications

Alternate Product Name: NKCC2

Product Clone Name: 4H4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 80-172

Epitope Target: NKCC2

Epitope Species: human

Product Reactivity/Detection: NKCC2

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, ELISA

Vialing Information: Each vial contains 100 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for NKCC2 Antibody (4H4): N/A