NK 2B4 CRISPR/Cas9 KO Plasmid (m): sc-421915
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- NK 2B4 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the NK 2B4 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
NK 2B4 CRISPR/Cas9 KO Plasmid (m) | sc-421915 | 20 µg | $397.00 |
Overview
Cd244 encodes NK 2B4 (CD244), a SLAM family immunoreceptor expressed on mouse natural killer cells and subsets of T cells that modulates cytotoxicity and cytokine production through interactions with CD48. Signaling is mediated by cytoplasmic ITSM motifs that recruit adaptor proteins such as SAP/SH2D1A or EAT-2, shaping downstream phosphorylation networks that tune activating versus inhibitory outputs. Through these pathways, CD244 contributes to immune synapse formation, target cell recognition, and regulation of inflammatory responses. Altered CD244 signaling has been linked to dysregulated antiviral and antitumor immunity, NK cell functional exhaustion, and immune imbalance in settings of chronic inflammation and hematologic malignancy models.
NK 2B4 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Cd244 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Cd244 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Cd244 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish NK 2B4 protein expression.
This CRISPR knockout system enables efficient generation of Cd244-deficient cell models for investigation of NK 2B4 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Cd244 exon(s) critical for NK 2B4 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Cd244 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by NK 2B4 CRISPR/Cas9 KO Plasmid (m) and NK 2B4 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Cd244 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by NK 2B4 HDR Plasmid (m) and NK 2B4 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Cd244 homology arms to support homology-directed repair at defined Cd244 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.