NIS Antibody (G-5): sc-514487

NIS Antibody (G-5) is a mouse monoclonal IgG3 kappa light chain antibody that detects NIS protein of mouse origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-NIS antibody (G-5) is available as the non-conjugated form. The sodium/iodide symporter (NIS) is an integral plasma membrane glycoprotein crucial for active iodide transport, primarily in the thyroid gland, but also in salivary glands, gastric mucosa, and lactating mammary gland. NIS localization in the plasma membrane facilitates iodide uptake, essential for synthesizing thyroid hormones that regulate metabolism, growth, and development. In lactating mammary gland, NIS plays a significant role by transporting iodide into milk, providing nursing infants with necessary iodide for their own thyroid hormone production. NIS expression is altered in various cancers; while expressed in some breast cancers, NIS levels are often reduced in most thyroid cancers, likely due to changes in binding activity of transcription factors such as AP2 and Sp1 to NIS promoter. NIS serves as a prerequisite for effective radioiodide treatment of thyroid cancer and a promising target for diagnostic and therapeutic strategies in breast cancer.

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NIS Antibody (G-5) References:

1. Hormone-dependent Polyploidy in the glandula orbitalis externa and glandula infraorbitalis of animals of different age.  |  Paulini, K. and Mohr, W. 1975. Beitr Pathol. 156: 65-74. PMID: 1180821
2. [The treatment, by imipramine, of the hemodynamic, functional and biochemical consequences of an experimental cranio-cervical trauma in rats].  |  Boismare, F., et al. 1976. C R Seances Soc Biol Fil. 170: 1110-7. PMID: 139985
3. Differential regulation of sodium/iodide symporter gene expression by nuclear receptor ligands in MCF-7 breast cancer cells.  |  Kogai, T., et al. 2005. Endocrinology. 146: 3059-69. PMID: 15817668
4. Expression of the sodium iodide symporter in differentiated thyroid cancer: clinical evidence.  |  Schmitz, G., et al. 2005. Nuklearmedizin. 44: 86-93. PMID: 15968416
5. Non-invasive imaging of cardiac transgene expression with PET: comparison of the human sodium/iodide symporter gene and HSV1-tk as the reporter gene.  |  Miyagawa, M., et al. 2005. Eur J Nucl Med Mol Imaging. 32: 1108-14. PMID: 15988610
6. Filariasis in West Kalimantan (Borneo), Indonesia.  |  Partona, F., et al. 1977. Southeast Asian J Trop Med Public Health. 8: 459-63. PMID: 26980
7. HMGB1-mediated autophagy regulates sodium/iodide symporter protein degradation in thyroid cancer cells.  |  Chai, W., et al. 2019. J Exp Clin Cancer Res. 38: 325. PMID: 31331356
8. The sodium iodide symporter (NIS) as theranostic gene: its emerging role in new imaging modalities and non-viral gene therapy.  |  Kitzberger, C., et al. 2022. EJNMMI Res. 12: 25. PMID: 35503582
9. Structural insights into the mechanism of the sodium/iodide symporter.  |  Ravera, S., et al. 2022. Nature. 612: 795-801. PMID: 36517601
10. Sodium iodide symporter-targeted gene therapy in glioblastoma.  |  Nicola, JP. and LaRocca, CJ. 2023. Mol Ther Oncolytics. 28: 44-45. PMID: 36654785
11. Sodium-iodide symporter and its related solute carriers in thyroid cancer.  |  Gong, Z., et al. 2024. J Endocrinol. 261: PMID: 38329368
12. Serum LH and FSH levels in the pregnant rabbit.  |  Osteen, KG. and Mills, TM. 1979. Proc Soc Exp Biol Med. 162: 454-7. PMID: 515029

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-NIS Antibody (G-5) has 3 citations in a variety of scientific publications.

Primary antibodies like Anti-NIS Antibody (G-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

NIS Antibody (G-5) Specifications

Alternate Product Name: NIS

Product Clone Name: G-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₃ kappa light chain

Amino Acids: 594-617

Epitope Target: NIS

Epitope Species: mouse

Product Reactivity/Detection: NIS

Species Reactivity/Detection: mouse

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₃ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 3 citations in various scientific publications

Available Conjugates/Forms for NIS Antibody (G-5): P: Blocking Peptide