NIPBL Antibody (C-9): sc-374625

NIPBL Antibody (C-9) is a mouse monoclonal IgG1 kappa light chain antibody that detects NIPBL protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-NIPBL antibody (C-9) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. NIPBL, also known as Nipped-B-like protein, IDN3, Delangin, or CDLS, is a crucial 2,804 amino acid nuclear protein that serves as the mammalian homolog of the Drosophila Nipped-B gene product. NIPBL plays a vital role in developmental regulation by facilitating communication between promoters and transcriptional enhancers, which is essential for proper gene expression during development. NIPBL is widely expressed, with particularly high levels found in skeletal muscle, heart, liver, and kidney, and contains five HEAT repeats that are important for structural integrity and function. NIPBL interacts with heterochromatin protein 1 alpha (HP1α), which may be significant for sister chromatid adhesion and maintaining proper chromatin structure. NIPBL exists in three isoforms and undergoes DNA damage-dependent phosphorylation, likely mediated by ATM or ATR kinases. Mutations in NIPBL are linked to Cornelia de Lange syndrome type 1 (CDLS1), a developmental disorder characterized by facial dysmorphisms, limb malformations, growth delays, cognitive impairments, and various organ system anomalies, underscoring NIPBL′s critical role in development and health.

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NIPBL Antibody (C-9) References:

1. Exclusion of linkage to the CDL1 gene region on chromosome 3q26.3 in some familial cases of Cornelia de Lange syndrome.  |  Krantz, ID., et al. 2001. Am J Med Genet. 101: 120-9. PMID: 11391654
2. Drosophila nipped-B protein supports sister chromatid cohesion and opposes the stromalin/Scc3 cohesion factor to facilitate long-range activation of the cut gene.  |  Rollins, RA., et al. 2004. Mol Cell Biol. 24: 3100-11. PMID: 15060134
3. NIPBL, encoding a homolog of fungal Scc2-type sister chromatid cohesion proteins and fly Nipped-B, is mutated in Cornelia de Lange syndrome.  |  Tonkin, ET., et al. 2004. Nat Genet. 36: 636-41. PMID: 15146185
4. Cornelia de Lange syndrome is caused by mutations in NIPBL, the human homolog of Drosophila melanogaster Nipped-B.  |  Krantz, ID., et al. 2004. Nat Genet. 36: 631-5. PMID: 15146186
5. NIPBL mutational analysis in 120 individuals with Cornelia de Lange syndrome and evaluation of genotype-phenotype correlations.  |  Gillis, LA., et al. 2004. Am J Hum Genet. 75: 610-23. PMID: 15318302
6. NIPBL mutations and genetic heterogeneity in Cornelia de Lange syndrome.  |  Borck, G., et al. 2004. J Med Genet. 41: e128. PMID: 15591270
7. Father-to-daughter transmission of Cornelia de Lange syndrome caused by a mutation in the 5' untranslated region of the NIPBL Gene.  |  Borck, G., et al. 2006. Hum Mutat. 27: 731-5. PMID: 16799922
8. Large genomic rearrangements in NIPBL are infrequent in Cornelia de Lange syndrome.  |  Bhuiyan, ZA., et al. 2007. Eur J Hum Genet. 15: 505-8. PMID: 17264868
9. The effect of Nipped-B-like (Nipbl) haploinsufficiency on genome-wide cohesin binding and target gene expression: modeling Cornelia de Lange syndrome.  |  Newkirk, DA., et al. 2017. Clin Epigenetics. 9: 89. PMID: 28855971
10. Downregulation of Cohesin Loading Factor Nipped-B-Like Protein (NIPBL) Induces Cell Cycle Arrest, Apoptosis, and Autophagy of Breast Cancer Cell Lines.  |  Zhou, H., et al. 2017. Med Sci Monit. 23: 4817-4825. PMID: 28987049
11. Cryo-EM structure of the human cohesin-NIPBL-DNA complex.  |  Shi, Z., et al. 2020. Science. 368: 1454-1459. PMID: 32409525
12. Nipped-B-like Protein Sensitizes Esophageal Squamous Cell Carcinoma Cells to Cisplatin via Upregulation of PUMA.  |  Zhang, S., et al. 2020. Technol Cancer Res Treat. 19: 1533033820960726. PMID: 33034274

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-NIPBL Antibody (C-9) has 22 citations in a variety of scientific publications.

Primary antibodies like Anti-NIPBL Antibody (C-9) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

NIPBL Antibody (C-9) Specifications

Alternate Product Name: NIPBL

Product Clone Name: C-9

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-300

Epitope Target: NIPBL

Epitope Species: human

Product Reactivity/Detection: NIPBL

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 22 citations in various scientific publications

Available Conjugates/Forms for NIPBL Antibody (C-9): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin