Date published: 2026-1-13

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NIF3L1 Antibody (B-8): sc-393632

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Datasheets
  • NIF3L1 Antibody (B-8) is a mouse monoclonal IgG2a κ NIF3L1 antibody provided at 200 µg/ml
  • raised against amino acids 23-146 mapping near the N-terminus of NIF3L1 of human origin
  • NIF3L1 Antibody (B-8) is recommended for detection of NIF3L1 of mouse, rat and human origin by WB, IP, IF and ELISA
  • Anti-NIF3L1 Antibody (B-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
  • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for NIF3L1 Antibody (B-8) for WB applications. These reagents are now offered in bundles with NIF3L1 Antibody (B-8) (see ordering information below).

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    SEE ALSO...

    NIF3L1 Antibody (B-8) is a mouse monoclonal IgG2a kappa light chain antibody that detects NIF3L1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). NIF3L1 Antibody (B-8) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. NGG1 interacting factor 3-like 1 (NIF3L1) is a 377 amino acid protein predominantly located in the cytoplasm, playing a crucial role in cellular processes. NIF3L1′s evolutionary conservation from bacteria to mammals underscores its importance in fundamental biological functions. NIF3L1 is involved in the retinoic acid-primed neural differentiation of P19 embryonic carcinoma cells, where NIF3L1 collaborates with Trip15/CSN2, a transcriptional corepressor and component of the COP9 signalosome. Additionally, NIF3L1 has been shown to interact with itself and with NIF3L1 binding protein 1 (NIF3L1 BP1), a novel protein believed to contain a leucine zipper domain, which may suggest a role in protein-protein interactions that are vital for cellular signaling and differentiation pathways.

    For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

    Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

    LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences

    NIF3L1 Antibody (B-8) References:

    1. Isolation and characterization of a novel human gene, NIF3L1, and its mouse ortholog, Nif3l1, highly conserved from bacteria to mammals.  |  Tascou, S., et al. 2000. Cytogenet Cell Genet. 90: 330-6. PMID: 11124544
    2. Cloning and characterization of three novel genes, ALS2CR1, ALS2CR2, and ALS2CR3, in the juvenile amyotrophic lateral sclerosis (ALS2) critical region at chromosome 2q33-q34: candidate genes for ALS2.  |  Hadano, S., et al. 2001. Genomics. 71: 200-13. PMID: 11161814
    3. The role of transcriptional corepressor Nif3l1 in early stage of neural differentiation via cooperation with Trip15/CSN2.  |  Akiyama, H., et al. 2003. J Biol Chem. 278: 10752-62. PMID: 12522100
    4. Identification and characterization of NIF3L1 BP1, a novel cytoplasmic interaction partner of the NIF3L1 protein.  |  Tascou, S., et al. 2003. Biochem Biophys Res Commun. 309: 440-8. PMID: 12951069
    5. The subcellular localization of the ChoRE-binding protein, encoded by the Williams-Beuren syndrome critical region gene 14, is regulated by 14-3-3.  |  Merla, G., et al. 2004. Hum Mol Genet. 13: 1505-14. PMID: 15163635
    6. Gene expression in mouse spermatogenesis during ontogenesis.  |  Giuffrida, V., et al. 2006. Int J Mol Med. 17: 523-8. PMID: 16465402
    7. Structure/functional properties of the yeast dual regulator protein NGG1 that are required for glucose repression.  |  Brandl, CJ., et al. 1996. J Biol Chem. 271: 9298-306. PMID: 8621592

    Ordering Information

    Product NameCatalog #UNITPriceQtyFAVORITES

    NIF3L1 Antibody (B-8)

    sc-393632
    200 µg/ml
    $322.00

    NIF3L1 Antibody (B-8): m-IgG Fc BP-HRP Bundle

    sc-526263
    200 µg Ab; 10 µg BP
    $361.00

    NIF3L1 Antibody (B-8): m-IgG2a BP-HRP Bundle

    sc-546546
    200 µg Ab; 10 µg BP
    $361.00

    NIF3L1 Antibody (B-8) AC

    sc-393632 AC
    500 µg/ml, 25% agarose
    $424.00

    NIF3L1 Antibody (B-8) HRP

    sc-393632 HRP
    200 µg/ml
    $322.00

    NIF3L1 Antibody (B-8) FITC

    sc-393632 FITC
    200 µg/ml
    $336.00

    NIF3L1 Antibody (B-8) PE

    sc-393632 PE
    200 µg/ml
    $349.00

    NIF3L1 Antibody (B-8) Alexa Fluor® 488

    sc-393632 AF488
    200 µg/ml
    $364.00

    NIF3L1 Antibody (B-8) Alexa Fluor® 546

    sc-393632 AF546
    200 µg/ml
    $364.00

    NIF3L1 Antibody (B-8) Alexa Fluor® 594

    sc-393632 AF594
    200 µg/ml
    $364.00

    NIF3L1 Antibody (B-8) Alexa Fluor® 647

    sc-393632 AF647
    200 µg/ml
    $364.00

    NIF3L1 Antibody (B-8) Alexa Fluor® 680

    sc-393632 AF680
    200 µg/ml
    $364.00

    NIF3L1 Antibody (B-8) Alexa Fluor® 790

    sc-393632 AF790
    200 µg/ml
    $364.00

    I am using the LiCor/Odyssey system. Which secondary antibody do you recommend using with NIF3L1 (B-8): sc-393632?

    Asked by: Dr Ninau Qelp
    We recommend using one of our our exclusive Mouse IgG Binding Proteins conjugated to CFL 680 or CFL 790. They are suitable for use with Near-Infrared (NIR) detection systems, such as LI-COR/Odyssey and other comparable systems. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
    Answered by: Technical Support
    Date published: 2017-02-27
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    Rated 5 out of 5 by from Very good Western blot data of NIF3L1 expressionVery good Western blot data of NIF3L1 expression in NTERA-2 cl.D1, Hep G2 and LNCaP whole cell lysates and human heart tissue extract . -SCBT QC
    Date published: 2014-07-09
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    NIF3L1 Antibody (B-8) is rated 5.0 out of 5 by 1.
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