Nidogen Antibody (JF4): sc-58476

Nidogen Antibody (JF4) is a rat monoclonal IgG2b antibody that detects Nidogen in mouse, rat, and human samples through applications such as western blotting (WB) and immunoprecipitation (IP). Nidogen, a crucial component of the extracellular matrix, plays a significant role in the structural integrity and function of basement membranes, which are essential for tissue organization and cellular signaling. Anti-Nidogen antibody (JF4) recognizes a vital linker between other extracellular matrix components, such as Laminin and Collagen Type IV, facilitating the assembly and stability of basement membranes. Nidogen′s importance extends to cellular adhesion processes, particularly in mesenchymal peritubular and myoepithelial cells, where Nidogen influences cell behavior in an autocrine manner. Additionally, Nidogen is characterized by a high level of glycosylation, which is critical for interactions with other matrix proteins, enhancing functional versatility. Notably, Nidogen is expressed during embryonic and fetal development, specifically in fully developed basement membranes of the ectoderm, highlighting an essential role in early tissue formation and development. Nidogen (JF4) antibody serves as an invaluable tool for researchers studying the dynamics of extracellular matrix interactions and their implications in developmental biology and tissue engineering.

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Nidogen Antibody (JF4) References:

1. Renal cell carcinomas and pancreatic adenocarcinomas produce nidogen in vitro and in vivo.  |  Oivula, J., et al. 1999. J Pathol. 187: 455-61. PMID: 10398106
2. Expression of the nidogen-binding site of the laminin gamma1 chain disturbs basement membrane formation and maintenance in F9 embryoid bodies.  |  Tunggal, J., et al. 2003. J Cell Sci. 116: 803-12. PMID: 12571278
3. Genome-wide association study identifies nidogen 1 (NID1) as a susceptibility locus to cutaneous nevi and melanoma risk.  |  Nan, H., et al. 2011. Hum Mol Genet. 20: 2673-9. PMID: 21478494
4. Posterior stromal cell apoptosis triggered by mechanical endothelial injury and basement membrane component nidogen-1 production in the cornea.  |  Medeiros, CS., et al. 2018. Exp Eye Res. 172: 30-35. PMID: 29596850
5. Temporal characterization of optic fissure basement membrane composition suggests nidogen may be an initial target of remodeling.  |  Carrara, N., et al. 2019. Dev Biol. 452: 43-54. PMID: 31034836
6. The role of Ca2+ binding in the self-aggregation of laminin-nidogen complexes.  |  Paulsson, M. 1988. J Biol Chem. 263: 5425-30. PMID: 3128539
7. Dynamics, structure and assembly of the basement membrane in developing salivary glands revealed by an exogenous EGFP-tagged nidogen probe.  |  Kadoya, Y., et al. 2022. Microscopy (Oxf). 71: 357-363. PMID: 35950724
8. Skin fibroblasts are the only source of nidogen during early basal lamina formation in vitro.  |  Fleischmajer, R., et al. 1995. J Invest Dermatol. 105: 597-601. PMID: 7561165
9. Sites of nidogen cleavage by proteases involved in tissue homeostasis and remodelling.  |  Mayer, U., et al. 1993. Eur J Biochem. 217: 877-84. PMID: 8223643
10. Mapping of nidogen binding sites for collagen type IV, heparan sulfate proteoglycan, and zinc.  |  Reinhardt, D., et al. 1993. J Biol Chem. 268: 10881-7. PMID: 8496153

The preferred Nidogen antibody is Nidogen Antibody (C-7): sc-133175.