Nidogen Antibody (JF2): sc-57010
- Nidogen Antibody (JF2) is a rat monoclonal IgG2a provided at 200 µg/ml
- raised against recombinant Nidogen of mouse origin
- recommended for detection of native and denatured Nidogen of mouse, rat and human origin by WB and IP
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for Nidogen Antibody (JF2). This work is in progress.
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Nidogen Antibody (JF2) is a rat monoclonal IgG2a antibody that detects Nidogen in mouse, rat, and human samples through western blotting (WB) and immunoprecipitation (IP) applications. Nidogen, also known as Entactin, is a crucial sulfated glycoprotein that plays a significant role in the formation and stability of basement membranes, which are essential for tissue architecture and cellular organization. Anti-Nidogen antibody (JF2) recognizes a vital linker between key extracellular matrix components, specifically Laminin 1 and Collagen Type IV, facilitating their assembly into a functional matrix. Nidogen′s importance extends beyond structural support; this protein is involved in regulating cellular adhesion and signaling processes, particularly in mesenchymal peritubular and myoepithelial cells, where Nidogen influences cell behavior in an autocrine manner. Notably, Nidogen is highly conserved across species, and Nidogen expression is tightly regulated during embryonic and fetal development, being present exclusively in fully developed ectodermal basement membranes. This specificity underscores Nidogen′s critical role in developmental biology and tissue integrity, making Nidogen (JF2) monoclonal antibody an invaluable tool for researchers studying extracellular matrix dynamics and related cellular processes.
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LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Nidogen Antibody (JF2) References:
- Complex between nidogen and laminin fragments reveals a paradigmatic beta-propeller interface. | Takagi, J., et al. 2003. Nature. 424: 969-74. PMID: 12931195
- Activity of MMP-19 inhibits capillary-like formation due to processing of nidogen-1. | Titz, B., et al. 2004. Cell Mol Life Sci. 61: 1826-33. PMID: 15241558
- Renal entactin (nidogen): isolation, characterization and tissue distribution. | Katz, A., et al. 1991. Kidney Int. 40: 643-52. PMID: 1745013
- Impaired wound healing in mice lacking the basement membrane protein nidogen 1. | Baranowsky, A., et al. 2010. Matrix Biol. 29: 15-21. PMID: 19766719
- IL-1 and TGF-β Modulation of Epithelial Basement Membrane Components Perlecan and Nidogen Production by Corneal Stromal Cells. | Saikia, P., et al. 2018. Invest Ophthalmol Vis Sci. 59: 5589-5598. PMID: 30480706
- Acute changes in nidogen-1 expression in the epidermal basement membrane of a 3-dimensional cultured human skin model after ultraviolet B irradiation. | Hirakawa, Y., et al. 2020. Photodermatol Photoimmunol Photomed. 36: 499-502. PMID: 32920911
- Entactin/nidogen: synthesis by bovine corneal endothelial cells and distribution in the human cornea. | Katz, A., et al. 1994. Invest Ophthalmol Vis Sci. 35: 495-502. PMID: 8112999
- Differential expression of laminin, nidogen and collagen IV genes in the midgestation mouse placenta. | Thomas, T. and Dziadek, M. 1993. Placenta. 14: 701-13. PMID: 8153091
- Laser scanning confocal examination and comparison of nidogen (entactin) with laminin in term human amniochorion. | Smith, J. and Ockleford, CD. 1994. Placenta. 15: 95-106. PMID: 8208674
- The laminin-nidogen complex is a ligand for a specific splice isoform of the transmembrane protein tyrosine phosphatase LAR. | O'Grady, P., et al. 1998. J Cell Biol. 141: 1675-84. PMID: 9647658
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Nidogen Antibody (JF2) | sc-57010 | 200 µg/ml | $322.00 |