Nidogen Antibody (ELM1): sc-33706
- Nidogen Antibody (ELM1) is a rat monoclonal IgG2a, cited in 16 publications, provided at 200 µg/ml
- raised against partially purified preparation of laminin from the EHS mouse tumor
- Nidogen Antibody (ELM1) is recommended for detection of Nidogen of mouse origin by IF and IHC(P); non cross-reactive with human Nidogen
- Anti-Nidogen Antibody (ELM1) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for Nidogen Antibody (ELM1). This work is in progress.
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Nidogen Antibody (ELM1) is a rat monoclonal IgG2a antibody that detects Nidogen of mouse origin by immunofluorescence (IF) and immunohistochemistry with paraffin-embedded sections (IHCP). Nidogen (ELM1) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Nidogen, also known as Entactin, is a crucial sulfated glycoprotein that plays a significant role in the structural integrity of basement membranes, which are essential extracellular matrices formed during embryogenesis. These membranes serve as a scaffold for various cellular components, including laminins and collagen IV, facilitating proper tissue organization and function. The interaction of Nidogen with laminin and collagen IV is vital for the assembly and stability of basement membranes, which are critical for processes such as cell adhesion, migration, and differentiation. Notably, Nidogen is highly conserved across species and exists in two isoforms, with Nidogen-2 exhibiting extensive N- and O-glycosylation. This post-translational modification influences Nidogen′s interactions with other extracellular matrix components, such as collagen types I and IV, and perlecan, thereby enhancing the structural and functional properties of the basement membrane. Nidogen is predominantly expressed in fully developed basement membranes of ectodermal origin during embryonic and fetal development, highlighting its specific role in tissue formation and maintenance.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Nidogen Antibody (ELM1) References:
- Nidogen and nidogen-associated basement membrane proteins and neuronal plasticity. | Köhling, R., et al. 2006. Neurodegener Dis. 3: 56-61. PMID: 16909038
- Skin basement membrane: the foundation of epidermal integrity--BM functions and diverse roles of bridging molecules nidogen and perlecan. | Breitkreutz, D., et al. 2013. Biomed Res Int. 2013: 179784. PMID: 23586018
- Fibrin with Laminin-Nidogen Reduces Fibrosis and Improves Soft Palate Regeneration Following Palatal Injury. | Rosero Salazar, DH., et al. 2021. Biomolecules. 11: PMID: 34680180
- Nidogen: A matrix protein with potential roles in musculoskeletal tissue regeneration. | Zhou, S., et al. 2022. Genes Dis. 9: 598-609. PMID: 35782975
- Visualization of basement membranes by a nidogen-based fluorescent reporter in mice. | Futaki, S., et al. 2023. Matrix Biol Plus. 18: 100133. PMID: 37131404
- Nidogen-1/NID1 Function and Regulation during Progression and Metastasis of Colorectal Cancer. | Rokavec, M., et al. 2023. Cancers (Basel). 15: PMID: 38001576
- Nidogen in development and disease. | Töpfer, U. and Holz, A. 2024. Front Cell Dev Biol. 12: 1380542. PMID: 38550383
- Temporally resolved proteomics identifies nidogen-2 as a cotarget in pancreatic cancer that modulates fibrosis and therapy response. | Pereira, BA., et al. 2024. Sci Adv. 10: eadl1197. PMID: 38959305
- Role of laminin-nidogen complexes in basement membrane formation during embryonic development. | Dziadek, M. 1995. Experientia. 51: 901-13. PMID: 7556571
- Structural and genetic analysis of laminin-nidogen interaction. | Mayer, U., et al. 1998. Ann N Y Acad Sci. 857: 130-42. PMID: 9917838
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Nidogen Antibody (ELM1) | sc-33706 | 200 µg/ml | $322.00 | |||
Nidogen Antibody (ELM1) AC | sc-33706 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Nidogen Antibody (ELM1) HRP | sc-33706 HRP | 200 µg/ml | $322.00 | |||
Nidogen Antibody (ELM1) FITC | sc-33706 FITC | 200 µg/ml | $336.00 | |||
Nidogen Antibody (ELM1) PE | sc-33706 PE | 200 µg/ml | $349.00 | |||
Nidogen Antibody (ELM1) Alexa Fluor® 488 | sc-33706 AF488 | 200 µg/ml | $364.00 | |||
Nidogen Antibody (ELM1) Alexa Fluor® 546 | sc-33706 AF546 | 200 µg/ml | $364.00 | |||
Nidogen Antibody (ELM1) Alexa Fluor® 594 | sc-33706 AF594 | 200 µg/ml | $364.00 | |||
Nidogen Antibody (ELM1) Alexa Fluor® 647 | sc-33706 AF647 | 200 µg/ml | $364.00 | |||
Nidogen Antibody (ELM1) Alexa Fluor® 680 | sc-33706 AF680 | 200 µg/ml | $364.00 | |||
Nidogen Antibody (ELM1) Alexa Fluor® 790 | sc-33706 AF790 | 200 µg/ml | $364.00 |