Nidogen-2 Antibody (A-7): sc-373859

Nidogen-2 Antibody (A-7) is a mouse monoclonal IgM antibody that detects Nidogen-2 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-Nidogen-2 antibody (A-7) is available as a non-conjugated format. Nidogen-2 plays a crucial role in basement membrane structural integrity, which is essential for tissue organization and function. Nidogen-2 is primarily located in the extracellular matrix, serving as a key connector between Laminin and Collagen IV networks, thereby facilitating protein integration into the membrane. Proper localization of Nidogen-2 is vital for maintaining basement membrane stability, which is critical for processes such as cell adhesion, migration, and differentiation during embryogenesis. Nidogen-2 interacts with other proteins, including Collagen I, Collagen IV, and Perlecan, to enhance tissue structural framework. Glycosylation at nitrogen and oxygen sites further influences Nidogen-2 function and interactions, making Nidogen-2 a significant player in the development and maintenance of various organs, including the heart, placenta, and bone.

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Nidogen-2 Antibody (A-7) References:

1. Altered synthesis of laminin 1 and absence of basement membrane component deposition in (beta)1 integrin-deficient embryoid bodies.  |  Aumailley, M., et al. 2000. J Cell Sci. 113 Pt 2: 259-68. PMID: 10633077
2. Nidogen-1. Expression and ultrastructural localization during the onset of mesoderm formation in the early mouse embryo.  |  Miosge, N., et al. 2000. J Histochem Cytochem. 48: 229-38. PMID: 10639489
3. Nidogen-1 regulates laminin-1-dependent mammary-specific gene expression.  |  Pujuguet, P., et al. 2000. J Cell Sci. 113 (Pt 5): 849-58. PMID: 10671374
4. Mesenchymal entactin-1 (nidogen-1) is required for adhesion of peritubular cells of the rat testis in vitro.  |  Konrad, L., et al. 2000. Eur J Cell Biol. 79: 112-20. PMID: 10727019
5. Ultrastructural colocalization of nidogen-1 and nidogen-2 with laminin-1 in murine kidney basement membranes.  |  Miosge, N., et al. 2000. Histochem Cell Biol. 113: 115-24. PMID: 10766264
6. The absence of nidogen 1 does not affect murine basement membrane formation.  |  Murshed, M., et al. 2000. Mol Cell Biol. 20: 7007-12. PMID: 10958695
7. Interaction of mouse thymocytes and a thymocyte-like cell line with the ECM glycoprotein entactin.  |  Schroen, DJ. and Cheung, HT. 1996. Cell Immunol. 167: 141-9. PMID: 8548838
8. Nidogen-2: a new basement membrane protein with diverse binding properties.  |  Kohfeldt, E., et al. 1998. J Mol Biol. 282: 99-109. PMID: 9733643

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Nidogen-2 Antibody (A-7) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-Nidogen-2 Antibody (A-7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Nidogen-2 Antibody (A-7) Specifications

Alternate Product Name: Nidogen-2

Product Clone Name: A-7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgM kappa light chain

Amino Acids: 211-510

Epitope Target: Nidogen-2

Epitope Species: mouse

Product Reactivity/Detection: Nidogen-2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgM kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for Nidogen-2 Antibody (A-7): N/A