Nicotinic Acetylcholine Receptor gamma/CHRNG Double Nickase Plasmid (h): sc-403321-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Nicotinic Acetylcholine Receptor gamma/CHRNG Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Nicotinic Acetylcholine Receptor gamma/CHRNG Double Nickase Plasmid (h) and Nicotinic Acetylcholine Receptor gamma/CHRNG Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CHRNG. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Nicotinic Acetylcholine Receptor gamma/CHRNG Antibody (66): sc-58597
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Nicotinic Acetylcholine Receptor gamma/CHRNG Double Nickase Plasmid (h) | sc-403321-NIC | 20 µg | $410.00 | |||
Nicotinic Acetylcholine Receptor gamma/CHRNG Double Nickase Plasmid (h2) | sc-403321-NIC-2 | 20 µg | $410.00 |
CHRNG encodes the gamma subunit of the muscle-type nicotinic acetylcholine receptor (nAChR), a ligand-gated ion channel that mediates fast synaptic transmission at the neuromuscular junction. During fetal and perinatal development, the gamma-containing receptor contributes to acetylcholine-dependent membrane depolarization, cation flux, and excitation–contraction coupling, supporting muscle maturation and motor innervation. CHRNG function is integrated with cholinergic signaling, postsynaptic membrane organization, and activity-dependent myofiber differentiation programs. Genetic variation or dysregulation of CHRNG has been associated with congenital neuromuscular disorders characterized by impaired fetal movement and abnormal neuromuscular junction formation, making it a useful target for studying developmental synaptogenesis and channel assembly.
Nicotinic Acetylcholine Receptor gamma/CHRNG Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CHRNG locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CHRNG. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CHRNG function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CHRNG-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.