Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Nicotinic Acetylcholine Receptor epsilon/CHRNE CRISPR/Cas9 KO Plasmid (m): sc-418960
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Nicotinic Acetylcholine Receptor epsilon/CHRNE CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Nicotinic Acetylcholine Receptor epsilon/CHRNE genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Nicotinic Acetylcholine Receptor epsilon/CHRNE Antibody (B-11): sc-376747
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Nicotinic Acetylcholine Receptor epsilon/CHRNE CRISPR/Cas9 KO Plasmid (m) | sc-418960 | 20 µg | $397.00 |
Overview
Chrne encodes the epsilon subunit of the muscle-type nicotinic acetylcholine receptor (nAChR), a ligand-gated ion channel concentrated at the postsynaptic membrane of the neuromuscular junction. Incorporation of CHRNE into the mature receptor supports synaptic transmission by enabling acetylcholine-evoked cation influx that depolarizes the muscle endplate and triggers excitation–contraction coupling. CHRNE function is tightly linked to synapse maturation and maintenance through pathways governing receptor clustering, endplate architecture, and activity-dependent signaling. Disruption of CHRNE is associated with congenital myasthenic syndromes and related neuromuscular transmission defects, making it relevant for mechanistic studies of motor function and synaptopathies.
Nicotinic Acetylcholine Receptor epsilon/CHRNE CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Chrne gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Chrne together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Chrne open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Nicotinic Acetylcholine Receptor epsilon/CHRNE protein expression.
This CRISPR knockout system enables efficient generation of Chrne-deficient cell models for investigation of Nicotinic Acetylcholine Receptor epsilon/CHRNE signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Chrne exon(s) critical for Nicotinic Acetylcholine Receptor epsilon/CHRNE function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Chrne genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Nicotinic Acetylcholine Receptor epsilon/CHRNE CRISPR/Cas9 KO Plasmid (m) and Nicotinic Acetylcholine Receptor epsilon/CHRNE CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Chrne locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Nicotinic Acetylcholine Receptor epsilon/CHRNE HDR Plasmid (m) and Nicotinic Acetylcholine Receptor epsilon/CHRNE HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Chrne homology arms to support homology-directed repair at defined Chrne target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.