Nicotinic Acetylcholine Receptor delta/CHRND Double Nickase Plasmid (h): sc-404001-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Nicotinic Acetylcholine Receptor delta/CHRND Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Nicotinic Acetylcholine Receptor delta/CHRND Double Nickase Plasmid (h) and Nicotinic Acetylcholine Receptor delta/CHRND Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CHRND. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Nicotinic Acetylcholine Receptor delta/CHRND Antibody (C-4): sc-390896
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Nicotinic Acetylcholine Receptor delta/CHRND Double Nickase Plasmid (h) | sc-404001-NIC | 20 µg | $410.00 | |||
Nicotinic Acetylcholine Receptor delta/CHRND Double Nickase Plasmid (h2) | sc-404001-NIC-2 | 20 µg | $410.00 |
CHRND encodes the delta subunit of the muscle-type nicotinic acetylcholine receptor (nAChR), a pentameric ligand-gated cation channel concentrated at the postsynaptic membrane of the neuromuscular junction. Upon acetylcholine binding, the receptor opens to permit Na⁺ and K⁺ flux, driving endplate depolarization and coupling synaptic transmission to skeletal muscle contraction. CHRND function is integrated with pathways governing synapse assembly and maturation, including agrin–LRP4–MuSK signaling, receptor clustering, and stabilization by rapsyn and associated scaffolds. Genetic perturbation of muscle nAChR subunits, including CHRND, is linked to disorders of neuromuscular transmission such as congenital myasthenic syndromes, making this gene relevant for mechanistic studies of synaptic excitability and receptor biogenesis.
Nicotinic Acetylcholine Receptor delta/CHRND Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CHRND locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CHRND. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CHRND function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CHRND-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.