Nicotinic Acetylcholine Receptor alpha 5/CHRNA5 Double Nickase Plasmid (h): sc-401702-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Nicotinic Acetylcholine Receptor alpha 5/CHRNA5 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Nicotinic Acetylcholine Receptor alpha 5/CHRNA5 Double Nickase Plasmid (h) and Nicotinic Acetylcholine Receptor alpha 5/CHRNA5 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CHRNA5. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Nicotinic Acetylcholine Receptor alpha 5/CHRNA5 Antibody (D-11): sc-376979
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Nicotinic Acetylcholine Receptor alpha 5/CHRNA5 Double Nickase Plasmid (h) | sc-401702-NIC | 20 µg | $410.00 | |||
Nicotinic Acetylcholine Receptor alpha 5/CHRNA5 Double Nickase Plasmid (h2) | sc-401702-NIC-2 | 20 µg | $410.00 |
CHRNA5 encodes the α5 accessory subunit of neuronal nicotinic acetylcholine receptors (nAChRs), assembling with other α and β subunits to modulate ligand-gated cation channel gating, Ca²⁺ permeability, and receptor desensitization. Through cholinergic signaling, α5-containing nAChRs influence membrane excitability and neurotransmitter release, linking to downstream calcium-dependent pathways and synaptic plasticity programs. Genetic variation and altered expression of CHRNA5 have been associated with nicotine dependence and smoking-related phenotypes, and have also been studied in the context of neuropsychiatric traits and airway epithelial biology relevant to smoking exposure. As a modulatory subunit, CHRNA5 provides a tractable handle for dissecting how receptor composition tunes cholinergic circuit function and stimulus-dependent transcriptional responses.
Nicotinic Acetylcholine Receptor alpha 5/CHRNA5 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CHRNA5 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CHRNA5. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CHRNA5 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CHRNA5-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.