NICE4 CRISPR/Cas9 KO Plasmid (h): sc-411379
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- NICE4 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the NICE4 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
NICE4 CRISPR/Cas9 KO Plasmid (h) | sc-411379 | 20 µg | $397.00 |
Overview
UBAP2L (NICE4) encodes an RNA-binding, ubiquitin-associated protein that localizes to cytoplasmic ribonucleoprotein granules and contributes to post-transcriptional control of gene expression. It has been implicated in stress granule assembly and RNA metabolism, supporting cellular adaptation to proteotoxic and oxidative stress while influencing proliferation and survival programs. Through these roles, UBAP2L connects to pathways governing mRNA translation and stability, and its dysregulation has been reported in studies of cancer-associated growth and metastatic phenotypes. Dissecting NICE4 function can help clarify how RNA granule dynamics and ubiquitin-related signaling coordinate cell state transitions relevant to disease biology.
NICE4 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the UBAP2L gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the UBAP2L together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the UBAP2L open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish NICE4 protein expression.
This CRISPR knockout system enables efficient generation of UBAP2L-deficient cell models for investigation of NICE4 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting UBAP2L exon(s) critical for NICE4 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple UBAP2L genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by NICE4 CRISPR/Cas9 KO Plasmid (h) and NICE4 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the UBAP2L locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by NICE4 HDR Plasmid (h) and NICE4 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by UBAP2L homology arms to support homology-directed repair at defined UBAP2L target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.