nicastrin Antibody (D-1): sc-376379

nicastrin Antibody (D-1) is a mouse monoclonal IgG2a antibody that detects nicastrin in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Nicastrin plays a crucial role in the assembly and function of the presenilin complex, which is essential for the regulated intramembrane proteolysis of various substrates, including the β-Amyloid precursor protein (βAPP) and Notch. The proper functioning of nicastrin is vital as nicastrin not only facilitates the processing of βAPP, thereby influencing the production of the neurotoxic β-Amyloid peptide associated with Familial Alzheimer′s disease, but also mediates Notch signaling, which is critical for cell differentiation and development. The structure of nicastrin includes hydrophilic amino and carboxy-terminal domains, a short hydrophobic transmembrane domain, and potential post-translational modification sites such as N-myristoylation and phosphorylation, which are important for stability and function. By understanding the intricate role of nicastrin in these pathways, researchers can explore therapeutic strategies aimed at modulating nicastrin activity to combat Alzheimer′s disease and other related disorders. anti-nicastrin antibody (D-1) is therefore an invaluable tool for studying the molecular mechanisms underlying these critical biological processes.

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nicastrin Antibody (D-1) References:

1. Nicastrin modulates presenilin-mediated notch/glp-1 signal transduction and betaAPP processing.  |  Yu, G., et al. 2000. Nature. 407: 48-54. PMID: 10993067
2. Functional reconstitution of gamma-secretase through coordinated expression of presenilin, nicastrin, Aph-1, and Pen-2.  |  Periz, G. and Fortini, ME. 2004. J Neurosci Res. 77: 309-22. PMID: 15248287
3. Nicastrin functions as a gamma-secretase-substrate receptor.  |  Shah, S., et al. 2005. Cell. 122: 435-47. PMID: 16096062
4. The Nicastrin ectodomain adopts a highly thermostable structure.  |  Fluhrer, R., et al. 2011. Biol Chem. 392: 995-1001. PMID: 21848507
5. Nicastrin and Notch4 drive endocrine therapy resistance and epithelial to mesenchymal transition in MCF7 breast cancer cells.  |  Lombardo, Y., et al. 2014. Breast Cancer Res. 16: R62. PMID: 24919951
6. MicroRNAs targeting Nicastrin regulate Aβ production and are affected by target site polymorphisms.  |  Delay, C., et al. 2014. Front Mol Neurosci. 7: 67. PMID: 25100943
7. Nicastrin/miR-30a-3p/RAB31 Axis Regulates Keratinocyte Differentiation by Impairing EGFR Signaling in Familial Acne Inversa.  |  He, Y., et al. 2019. J Invest Dermatol. 139: 124-134. PMID: 30120935
8. Essential requirement for nicastrin in marginal zone and B-1 B cell development.  |  Choi, JH., et al. 2020. Proc Natl Acad Sci U S A. 117: 4894-4901. PMID: 32071239
9. Validation of a commercial antibody to detect endogenous human nicastrin by immunoblot.  |  Mesa, RA. and Roberson, EDO. 2019. F1000Res. 8: 1211. PMID: 32399180
10. Nanoscale organization of Nicastrin, the substrate receptor of the γ-secretase complex, as independent molecular domains.  |  Kedia, S., et al. 2021. Mol Brain. 14: 158. PMID: 34645511

The preferred nicastrin antibody is nicastrin Antibody (B-3): sc-376513.