NGF Double Nickase Plasmid (h): sc-400292-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- NGF Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- NGF Double Nickase Plasmid (h) and NGF Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting NGF. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: NGF Antibody (B-4): sc-518166
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
NGF Double Nickase Plasmid (h) | sc-400292-NIC | 20 µg | $410.00 | |||
NGF Double Nickase Plasmid (h2) | sc-400292-NIC-2 | 20 µg | $410.00 |
Nerve growth factor (NGF) is a secreted neurotrophin that binds the TrkA (NTRK1) receptor tyrosine kinase and the p75 neurotrophin receptor (NGFR) to regulate neuronal survival, differentiation, and neurite outgrowth. NGF signaling engages MAPK/ERK, PI3K–AKT, and PLCγ pathways, coordinating transcriptional programs, cytoskeletal dynamics, and synaptic maintenance in peripheral and central nervous system contexts. Beyond neurodevelopment, NGF contributes to neuroimmune communication and can modulate nociceptor sensitization and inflammatory responses through receptor-dependent signaling. Dysregulated NGF expression or signaling has been linked to neurodegenerative processes, chronic pain mechanisms, and altered neural plasticity, making NGF a widely studied node in nervous system biology.
NGF Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the NGF locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within NGF. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt NGF function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of NGF-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.