NG2 Antibody (9.2.27): sc-80003

NG2 Antibody (9.2.27) is a mouse monoclonal IgG2a kappa light chain antibody that detects NG2 in human samples through immunoprecipitation (IP), immunofluorescence (IF), and flow cytometry (FCM) applications. Anti-NG2 antibody (9.2.27) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. NG2, also known as melanoma-associated chondroitin sulfate proteoglycan 4, MCSP, MCSPG, MSK16, and MEL-CSPG, plays a crucial role in stabilizing cell-substratum interactions during the early stages of melanoma cell spreading on endothelial basement membranes, which is vital for tumor progression. NG2 is primarily located on the surface of various cell types, including precursor and mature NG2 cells, as well as human melanoma, glioma, and proliferating brain endothelial cells. NG2′s presence on blood vessels throughout the rat embryo and in microvessels within the central nervous system highlights its importance in vascular development and maintenance. Additionally, NG2 acts as a co-receptor for spreading and focal contact formation in malignant melanoma cells, enhancing the activation of key signaling pathways that are critical for tumor invasion and growth. Notably, post-translational modifications, such as the phosphorylation of threonine 2256 in rat NG2 (threonine 2252 in human NG2), lead to significant changes in NG2′s distribution on the cell surface, promoting increased cell motility and polarization in astrocytomas. This multifaceted role of NG2 underscores its potential as a therapeutic target and a marker for various cellular processes in cancer biology.

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Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-NG2 Antibody (9.2.27) has 3 citations in a variety of scientific publications.

Primary antibodies like Anti-NG2 Antibody (9.2.27) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

NG2 Antibody (9.2.27) Specifications

Alternate Product Name: NG2

Product Clone Name: 9.2.27

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids:

Epitope Target: NG2

Epitope Species: human

Product Reactivity/Detection: precursor and mature NG2 and human melanoma, glioma and proliferating brain endothelial cells

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: IP, IF, FCM

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 3 citations in various scientific publications

Available Conjugates/Forms for NG2 Antibody (9.2.27): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred NG2 antibody is NG2 Antibody (LHM 2): sc-53389.